Hsu K-F, Huang S-C, Shiau A-L, Cheng Y-M, Shen M-R, Chen Y-F, Lin C-Y, Lee B-H, Chou C-Y
Department of Obstetrics and Gynecology, Chi Mei Foundation Hospital, Tainan, Taiwan.
Int J Gynecol Cancer. 2007 Jan-Feb;17(1):174-81. doi: 10.1111/j.1525-1438.2006.00663.x.
Squamous cell carcinoma antigen (SCCA) is a tumor marker for patients with squamous cell carcinoma of uterine cervix, lung, and esophagus. It was encoded by two highly homologous genes, SCCA1 and SCCA2. However, the relevance of SCCA genes to squamous cell carcinogenesis and patient outcome remains far from clear. In this study, by using laser microdissection and real-time quantitative polymerase chain reaction procedures, the messenger RNA (mRNA) expression of the SCCA1 and SCCA2 genes in normal, dysplastic, and malignant squamous epithelia from uterine cervical tissues were analyzed and correlated with outcome of cancer patients. We found that the SCCA2/A1 mRNA ratios were progressively increased from normal, dysplastic, to cancer cells, and the mean ratio was significantly higher in cancer tissues than that in normal epithelium (P= 0.02). The SCCA2/A1 mRNA ratios were not significantly associated with types of human papillomavirus infection (P > 0.05). High SCCA2/SCCA1 mRNA ratios (ratio >1) were an independent predictor of disease recurrence (relative risk: 3.58; P= 0.003). Of the 38 patients with cervical cancer, 12 patients with high SCCA2/SCCA1 mRNA ratios had a significant lower 2-year disease-free survival of only 50%, while it was 92% in those with low SCCA2/SCCA1 mRNA ratios (P < 0.001). In conclusion, our study indicated that the ratios of SCCA2 to SCCA1 RNA were increased during the process of cervical carcinogenesis, and patients with elevated SCCA2/A1 ratio carried a higher risk for recurrence in early-stage uterine cervical cancer.
鳞状细胞癌抗原（SCCA）是子宫颈、肺和食管鳞状细胞癌患者的一种肿瘤标志物。它由两个高度同源的基因SCCA1和SCCA2编码。然而，SCCA基因与鳞状细胞癌发生及患者预后的相关性仍远未明确。在本研究中，通过激光显微切割和实时定量聚合酶链反应程序，分析了子宫颈组织中正常、发育异常和恶性鳞状上皮中SCCA1和SCCA2基因的信使核糖核酸（mRNA）表达，并将其与癌症患者的预后相关联。我们发现，SCCA2/A1 mRNA比值从正常、发育异常到癌细胞逐渐升高，癌组织中的平均比值显著高于正常上皮（P = 0.02）。SCCA2/A1 mRNA比值与人乳头瘤病毒感染类型无显著关联（P > 0.05）。高SCCA2/SCCA1 mRNA比值（比值>1）是疾病复发的独立预测指标（相对风险：3.58；P = 0.003）。在38例宫颈癌患者中，12例SCCA2/SCCA1 mRNA比值高的患者2年无病生存率显著较低，仅为50%，而SCCA2/SCCA1 mRNA比值低的患者为92%（P < 0.001）。总之，我们的研究表明，在子宫颈癌发生过程中SCCA2与SCCA1 RNA的比值升高，SCCA2/A1比值升高的患者在早期子宫颈癌中复发风险更高。
