Department of Otorhinolaryngology, Head and Neck Surgery, University of the Ryukyus, Okinawa, Japan.
Cancer Sci. 2012 Dec;103(12):2127-34. doi: 10.1111/cas.12009. Epub 2012 Oct 17.
To clarify the synergistic influence of human papillomavirus (HPV) status and squamous cell carcinoma antigen (SCCA) mRNA expression on head and neck squamous cell carcinoma (HNSCC) prognosis, HPV DNA presence and SCCA1 and SCCA2 mRNA expression were determined by PCR and quantitative real-time RT-PCR, respectively, in 121 patients with primary HNSCC who were receiving curative treatment. HPV DNA was detected in 28.1% (34/121) of HNSCC cases, and only high-risk types (HPV-16, HPV-33, HPV-35 and HPV-58) were observed. Positive HPV status showed a significantly better prognosis than negative HPV status (P = 0.022). An elevated SCCA2/SCCA1 mRNA ratio was an independent predictor of disease recurrence (P = 0.004). In addition, HPV-negative patients with a high SCCA2/SCCA1 ratio (>0.27) had a significantly lower recurrence-free survival rate than HPV-negative patients with a low SCCA2/SCCA1 ratio (P < 0.011). Our findings revealed that both HPV status and the SCCA2/SCCA1 mRNA ratio are independently associated with prognosis in HNSCC. Patients with both a HPV-negative status and a high SCCA2/SCCA1 ratio might need intensified treatment and rigorous follow up after treatment because of the high risk of recurrence.
为了阐明人乳头瘤病毒（HPV）状态和鳞状细胞癌抗原（SCCA）mRNA 表达对头颈部鳞状细胞癌（HNSCC）预后的协同影响，我们通过聚合酶链反应（PCR）和实时定量 RT-PCR 分别检测了 121 例接受根治性治疗的原发性 HNSCC 患者的 HPV DNA 存在情况以及 SCCA1 和 SCCA2 mRNA 的表达情况。在 121 例 HNSCC 病例中，检测到 28.1%（34/121）的 HPV DNA，且仅观察到高危型（HPV-16、HPV-33、HPV-35 和 HPV-58）。HPV 阳性状态的预后明显优于 HPV 阴性状态（P = 0.022）。SCCA2/SCCA1 mRNA 比值升高是疾病复发的独立预测因子（P = 0.004）。此外，HPV 阴性且 SCCA2/SCCA1 比值较高（>0.27）的患者的无复发生存率明显低于 HPV 阴性且 SCCA2/SCCA1 比值较低的患者（P < 0.011）。我们的研究结果表明，HPV 状态和 SCCA2/SCCA1 mRNA 比值均与 HNSCC 的预后独立相关。HPV 阴性且 SCCA2/SCCA1 比值较高的患者可能需要强化治疗，并在治疗后进行严格的随访，因为复发风险较高。
