Farnia P, Masjedi M R, Nasiri B, Mirsaedi M, Sorooch S, Kazeampour M, Velayati A A
National Research Institute of Tuberculosis and Lung Disease, WHO Collaborating Centre of Tuberculosis, Darabad, Tehran, Iran.
Epidemiol Infect. 2007 Feb;135(2):346-52. doi: 10.1017/S0950268806006790.
The stability of IS6110 restriction fragment length polymorphism (RFLP) pattern was determined in 31 isolates from patients with multidrug-resistant tuberculosis (MDR-TB). These patients were in actual chains of transmission and they referred to the National Institute of Tuberculosis and Lung Diseases, Tehran, Iran. Susceptibility testing against first- and second-line drugs were performed by the proportional method on Lowenstein-Jensen culture media. Thereafter, DNA fingerprinting by IS6110 with direct repeat (DR) region as a probe was performed by standard protocols. The rate of IS6110 changes was 16%, although, no variation was found in the DR region, in a time-span of 1-63 months. The strains with unstable IS6110 patterns were resistant to all drugs tested, and the majority of them (60%) were collected from HIV-positive patients. The results demonstrated that for a reliable interpretation of strain typing, it is better to use an additional marker along with IS6110 RFLP.
在31株耐多药结核病（MDR-TB）患者的分离菌株中，测定了IS6110限制性片段长度多态性（RFLP）模式的稳定性。这些患者处于实际传播链中，他们转诊至伊朗德黑兰的国家结核病和肺部疾病研究所。在罗-琴培养基上采用比例法对一线和二线药物进行药敏试验。此后，按照标准方案，以直接重复序列（DR）区域为探针，通过IS6110进行DNA指纹分析。在1至63个月的时间跨度内，IS6110的变化率为16%，不过，在DR区域未发现变异。IS6110模式不稳定的菌株对所有测试药物均耐药，其中大多数（60%）菌株来自HIV阳性患者。结果表明，为了对菌株分型进行可靠的解读，最好在使用IS6110 RFLP的同时再使用一个额外的标记物。
