Weroński Konrad, Busquets M Antónia, Girona Victória, Prat Josefina
Physical Chemistry Department, Faculty of Pharmacy, University of Barcelona, Av. Joan XXIII s/n, 08028 Barcelona, Spain.
Colloids Surf B Biointerfaces. 2007 May 15;57(1):8-16. doi: 10.1016/j.colsurfb.2006.11.028. Epub 2006 Dec 1.
Two decapeptide fragments of the non-structural hepatitis G NS3 protein (GBV-C/HGV), 513-522 (RGRTGRGRSG) and 505-514 (SAELSMQRRG), as well as their palmitoylated derivatives were synthesized. The physico-chemical properties of the peptides were analyzed in both the absence and presence of the zwitterionic 1,2-dipalmitoyl-sn-glycero-3-phosphatidylcholine (DPPC), the negative 1,2-dipalmitoyl-sn-glycero-3-[phospho-rac-(1-glycerol)] (DPPG) and the positive 1,2-dioeloyl-3-trimethylammonium-propane (DOTAP) lipid monolayers. Based on their high hydrophilic properties, neither parent peptide presented surface activity and their incorporation into lipid monolayers was low. In contrast, their palmitoylated derivatives showed concentration-dependent surface activity and could be inserted into lipid monolayers to varying degrees depending on their sequence. Compression isotherms showed that the presence of palmitoylated peptides in the subphase resulted in a molecular arrangement less condensed than that corresponding to the pure phospholipid. In concordance with the monolayer results, differential scanning calorimetry (DSC) demonstrated that the parent peptides did not have any effect on the thermograms, while the palmitoylated derivatives affected the thermotropic properties of DPPC bilayers.
合成了丙型肝炎病毒G型非结构蛋白NS3（GBV-C/HGV）的两个十肽片段，即513 - 522（RGRTGRGRSG）和505 - 514（SAELSMQRRG），以及它们的棕榈酰化衍生物。在两性离子的1,2 - 二棕榈酰 - sn - 甘油 - 3 - 磷脂酰胆碱（DPPC）、带负电荷的1,2 - 二棕榈酰 - sn - 甘油 - 3 - [磷酸 - rac -（1 - 甘油）]（DPPG）和带正电荷的1,2 - 二油酰基 - 3 - 三甲基铵丙烷（DOTAP）脂质单层存在和不存在的情况下，分析了这些肽的物理化学性质。基于它们的高亲水性，两种亲本肽均未表现出表面活性，并且它们掺入脂质单层的比例较低。相反，它们的棕榈酰化衍生物表现出浓度依赖性的表面活性，并且根据其序列可以不同程度地插入脂质单层中。压缩等温线表明，亚相中存在棕榈酰化肽导致分子排列比相应的纯磷脂更疏松。与单层结果一致，差示扫描量热法（DSC）表明亲本肽对热谱图没有任何影响，而棕榈酰化衍生物影响了DPPC双层的热致性质。
