School of Chemical Sciences, The University of Auckland, Auckland, New Zealand.
School of Biological Sciences, The University of Auckland, Auckland, New Zealand.
Methods Mol Biol. 2021;2355:253-263. doi: 10.1007/978-1-0716-1617-8_19.
Lipidation of polypeptides with a fatty acid to form N-linked lipopeptides can be a time consuming process due to the need to mask other reactive function groups present on the side chains of amino acids. Cysteine Lipidation on a Peptide or Amino acid (CLipPA) technology enables the direct lipidation of unprotected peptides containing a free thiol group to afford S-lipidated lipopeptides. A generalized procedure for the synthesis of S-lipopeptides is described which facilities rapid preparation of tens of analogs of lipopeptides from a single thiolated polypeptide precursor.
将脂肪酸酰化多肽形成 N-连接的脂肽是一个耗时的过程，因为需要掩蔽氨基酸侧链上存在的其他反应性功能基团。肽或氨基酸的半胱氨酸脂化 (CLipPA) 技术可使含有游离巯基的未保护肽直接脂化，得到 S-脂肽。本文描述了一种 S-脂肽合成的通用方法，可从单个巯基化多肽前体快速制备数十种脂肽类似物。
