Ueno H, Ohya T, Ito H, Kobayashi Y, Yamada K, Sato M
Department of Clinical Veterinary Science, Obihiro University of Agriculture and Veterinary Medicine, Inada-cho, Obihiro 080-8555, Japan.
J Plast Reconstr Aesthet Surg. 2007;60(3):304-10. doi: 10.1016/j.bjps.2006.06.024. Epub 2006 Sep 18.
Radiation-impaired wounds are characterized by fibroblast and endothelial cell injury, resulting in delayed wound healing. Several previous studies have indicated that chitosan accelerates wound healing by up-regulating growth factor synthesis. In this study, the topical application of chitosan onto radiation-impaired wounds was investigated. An X-ray irradiated (25Gy) skin wound was treated with cotton fibre-type chitosan in dogs. Histopathologically, neovascularization was significantly accelerated in irradiated wounds in the chitosan application group (rad-chi group) when compared with irradiated wounds in the control group (rad-cont group). Vascular endothelial growth factor (VEGF) messenger ribonucleic acid (mRNA) expression in granulation tissue was positive in the rad-chi group, but was negative in the rad-cont group. The present results confirmed advanced granulation and capillary formation in wounds treated with chitosan, even after irradiation.
放射性损伤伤口的特征是成纤维细胞和内皮细胞损伤，导致伤口愈合延迟。先前的多项研究表明，壳聚糖可通过上调生长因子合成来加速伤口愈合。在本研究中，对壳聚糖局部应用于放射性损伤伤口进行了研究。用棉纤维型壳聚糖治疗犬的X射线照射（25Gy）皮肤伤口。组织病理学检查显示，与对照组（rad-cont组）的照射伤口相比，壳聚糖应用组（rad-chi组）的照射伤口新血管形成明显加速。肉芽组织中血管内皮生长因子（VEGF）信使核糖核酸（mRNA）表达在rad-chi组为阳性，而在rad-cont组为阴性。目前的结果证实，即使在照射后，用壳聚糖治疗的伤口中肉芽组织和毛细血管形成也得到了促进。
