Tuttosi S, Bretscher P A
Department of Microbiology, University of Saskatchewan, Saskatoon, Canada.
J Immunol. 1992 Jan 15;148(2):397-403.
We have developed an in vivo/in vitro immunization procedure with xenogeneic RBC as Ag that results in the generation of a lymphoid population that expresses potent delayed-type hypersensitivity (DTH) and produces little, if any, antibody. This lymphoid population contains Ag-specific CD8+ T cells that can inhibit the induction of a strong IgG response. These CD8+ T cells are shown to not only inhibit the antibody response in an Ag-specific manner but allow the Ag to induce cells of the target population to express DTH. Furthermore, the Ag-specific inhibition of the antibody response and the Ag-specific enhancement of the induction of DTH appear to be coordinately regulated, as the same number of CD8+ T cells cells is required to achieve both effects. Thus these CD8+ T cells are shown to switch the response induced by Ag from a humoral to a cell-mediated mode. These regulatory characteristics are consistent with a physiologic role for these cells of ensuring the absence of antibody production during a strong, cell-mediated response.
我们开发了一种以异种红细胞作为抗原的体内/体外免疫程序，该程序可导致产生一群表达强效迟发型超敏反应（DTH）且几乎不产生抗体的淋巴细胞群体。这群淋巴细胞包含抗原特异性CD8 + T细胞，它们能够抑制强烈的IgG反应的诱导。这些CD8 + T细胞不仅以抗原特异性方式抑制抗体反应，还能使抗原诱导靶细胞群体表达DTH。此外，抗体反应的抗原特异性抑制和DTH诱导的抗原特异性增强似乎是协同调节的，因为实现这两种效应所需的CD8 + T细胞数量相同。因此，这些CD8 + T细胞可将抗原诱导的反应从体液模式转变为细胞介导模式。这些调节特性与这些细胞在确保强烈的细胞介导反应期间不产生抗体方面的生理作用相一致。
