Carvalho Paulo de Tarso Camillo de, Marques Ana Paula da Costa, Reis Felipe Abdalla dos, Belchior Ana Carulina Guimarães, Silva Iandara Schettert, Habitante Carlos Alexandre, Sussai Daniela Aparecida
Department of Physiotherapy, UNIDERP, Brazil.
Acta Cir Bras. 2006;21 Suppl 4:32-5. doi: 10.1590/s0102-86502006001000008.
To evaluate in vitro the antibacterial effect of diode laser light of wavelength 650 nm, in association with the photosensitive substance toluidine blue, on the bacteria in infected skin ulcers.
Samples were collected by means of swabs containing a medium for transporting infected material from skin ulcers. The material was inoculated into culturing medium containing azide blood agar for the growth of Gram-positive bacteria, and MacConkey agar for Gram-negative bacteria, and incubated for 48 hours. The results obtained from counting the colony-forming units were correlated and subjected to statistical analysis, adopting the significance level of p > or = 0.05.
From analysis of variance (ANOVA), the result for the general mean was p = 0.0215. Using the t test with post-hoc test, the result for TBO vs. Control was p = 0.0186, and for TBO + Laser vs. Control it was p = 0.0039.
There was a significant reduction in colony-forming units when the cultures were subjected to photodynamic therapy.
在体外评估波长650nm的二极管激光与光敏物质甲苯胺蓝联合使用对感染性皮肤溃疡中细菌的抗菌作用。
通过含有用于运输皮肤溃疡感染物质的培养基的拭子收集样本。将该物质接种到含有用于革兰氏阳性菌生长的叠氮化物血琼脂和用于革兰氏阴性菌的麦康凯琼脂的培养基中，并孵育48小时。对通过计数菌落形成单位获得的结果进行关联并进行统计分析，采用p≥0.05的显著性水平。
通过方差分析（ANOVA），总体均值的结果为p = 0.0215。使用事后检验的t检验，TBO与对照组的结果为p = 0.0186，TBO +激光与对照组的结果为p = 0.0039。
当培养物接受光动力疗法时，菌落形成单位显著减少。
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