掺铒钇铝石榴石激光联合光动力疗法对种植体表面伴放线聚集杆菌生物膜的杀菌作用
Bactericidal Effect of Erbium-Doped Yttrium Aluminum Garnet Laser and Photodynamic Therapy on Aggregatibacter Actinomycetemcomitans Biofilm on Implant Surface.
作者信息
Saffarpour Anna, Fekrazad Reza, Heibati Maryam Naghavi, Bahador Abbas, Saffarpour Aida, Rokn Amir R, Iranparvar Aysel, KharaziFard Mohammad J
出版信息
Int J Oral Maxillofac Implants. 2016 May-Jun;31(3):e71-8. doi: 10.11607/jomi.4224.
PURPOSE
Peri-implantitis is a common complication of dental implants. The first step of treatment is elimination of bacterial biofilm and disinfection of the implant surface. This study sought to compare the effects of an erbium-doped yttrium aluminum garnet (Er:YAG) laser, photodynamic therapy using an indocyanin green-based photosensitizer (ICG-based PS) and diode laser, toluidine blue O (TBO) photosensitizer and light-emitting diode (LED) light source, and 2% chlorhexidine (CHX) on biofilm of Aggregatibacter actinomycetemcomitans to sandblasted, large-grit, acid-etched (SLA) implant surfaces.
MATERIALS AND METHODS
Fifty SLA implants were divided into five groups and were incubated with A actinomycetemcomitans bacteria to form bacterial biofilm. Group 1 underwent Er:YAG laser radiation (with 10-Hz frequency, 100-mJ energy, and 1-W power); group 2 was subjected to LED (with 630-nm wavelength and maximum output intensity of 2.000 to 4.000 mW/cm(2)) and TBO as a photosensitizer; group 3 was exposed to diode laser radiation (with 810-nm wavelength and 300-mW power) and ICG-based PS; and group 4 was immersed in 2% CHX. Group 5 was the control group, and the samples were rinsed with normal saline. The number of colony-forming units (CFU) per implant was then calculated. Data were analyzed using one-way analysis of variance (ANOVA), and the five groups were compared.
RESULTS
Significant differences was found between the control group and the other groups (P < .01). The lowest mean of CFU per implant count was in group 4 (P < .01), and the highest mean belonged to the control group. Photodynamic therapy by TBO + LED and ICG-based PS + diode laser was more effective than Er:YAG laser irradiation in suppression of this organism (P < .01). There was no significant difference between groups 2 and 3.
CONCLUSION
The antibacterial effect of 2% CHX was greater than that of other understudy methods.
目的
种植体周围炎是牙种植体常见的并发症。治疗的第一步是清除细菌生物膜并对种植体表面进行消毒。本研究旨在比较掺铒钇铝石榴石（Er:YAG）激光、使用基于吲哚菁绿的光敏剂（ICG基PS）的光动力疗法和二极管激光、甲苯胺蓝O（TBO）光敏剂和发光二极管（LED）光源，以及2%氯己定（CHX）对喷砂、大颗粒、酸蚀（SLA）种植体表面伴放线聚集杆菌生物膜的影响。
材料与方法
将50个SLA种植体分为五组，与伴放线聚集杆菌一起孵育以形成细菌生物膜。第1组接受Er:YAG激光照射（频率10Hz，能量100mJ，功率1W）；第2组使用LED（波长630nm，最大输出强度2.000至4.000mW/cm²）和TBO作为光敏剂；第3组接受二极管激光照射（波长810nm，功率300mW）和ICG基PS；第4组浸泡在2%CHX中。第5组为对照组，样品用生理盐水冲洗。然后计算每个种植体的菌落形成单位（CFU）数量。使用单因素方差分析（ANOVA）对数据进行分析，并比较五组。
结果
对照组与其他组之间存在显著差异（P<.01）。每个种植体CFU计数的最低平均值在第4组（P<.01），最高平均值属于对照组。TBO+LED和ICG基PS+二极管激光的光动力疗法在抑制这种微生物方面比Er:YAG激光照射更有效（P<.01）。第2组和第3组之间没有显著差异。
结论
2%CHX的抗菌效果大于其他所研究的方法。
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