Kim Kyung Il, Sunter Garry, Bisaro David M, Chung In Sik
Graduate School of Biotechnology and Plant Metabolism Research Center, Kyung Hee University, Suwon 449-701, Korea.
Plant Mol Biol. 2007 May;64(1-2):103-12. doi: 10.1007/s11103-007-9137-z. Epub 2007 Feb 9.
Recombinant green fluorescent protein (GFP) with a molecular mass of 29 kDa was transiently expressed in Agrobacterium-inoculated leaf-disks prepared from Nicotiana benthamiana plants. Expression of GFP from the Cauliflower mosaic virus (CaMV) 35 S promoter within a replicating vector based on the geminivirus Beet curly top virus (BCTV) was more than 3 times higher than from a control, non-replicating vector. Use of the Cassava vein mosaic virus (CsVMV) promoter in the BCTV replicating vector increased the expression of recombinant GFP 320% at the transcript level, compared to use of the control CaMV 35 S promoter. Expression of recombinant GFP from Agrobacterium-inoculated leaf-disks of N. benthamiana was further enhanced up to 240% in the presence of post-transcriptional gene silencing suppressor p19.
分子量为29 kDa的重组绿色荧光蛋白（GFP）在由本氏烟草植株制备的经农杆菌接种的叶盘中介导瞬时表达。基于双生病毒甜菜曲顶病毒（BCTV）的复制载体中，来自花椰菜花叶病毒（CaMV）35S启动子的GFP表达比对照非复制载体高3倍以上。与使用对照CaMV 35S启动子相比，在BCTV复制载体中使用木薯脉花叶病毒（CsVMV）启动子在转录水平上使重组GFP的表达增加了320%。在转录后基因沉默抑制因子p19存在的情况下，来自本氏烟草经农杆菌接种叶盘的重组GFP表达进一步提高了240%。
