Hefferon Kathleen L
Cell and Systems Biology, University of Toronto, Toronto, Ontario M5S 2J7, Canada.
Vaccines (Basel). 2014 Aug 5;2(3):642-53. doi: 10.3390/vaccines2030642.
Plants represent a safe, efficacious and inexpensive production platform by which to provide vaccines and other therapeutic proteins to the world's poor. Plant virus expression vector technology has rapidly become one of the most popular methods to express pharmaceutical proteins in plants. This review discusses several of the state-of-the-art plant expression systems based upon geminiviruses that have been engineered for vaccine production. An overview of the advantages of these small, single-stranded DNA viruses is provided and comparisons are made with other virus expression systems. Advances in the design of several different geminivirus vectors are presented in this review, and examples of vaccines and other biologics generated from each are described.
植物是一个安全、有效且廉价的生产平台，可借此为世界上的贫困人口提供疫苗和其他治疗性蛋白质。植物病毒表达载体技术已迅速成为在植物中表达药用蛋白质最受欢迎的方法之一。本综述讨论了几种基于双生病毒构建的、用于疫苗生产的先进植物表达系统。文中概述了这些小型单链DNA病毒的优势，并与其他病毒表达系统进行了比较。本综述介绍了几种不同双生病毒载体设计方面的进展，并描述了由每种载体生产的疫苗和其他生物制品的实例。
