Sohda T, Iwata K, Soejima H, Kamimura S, Shijo H, Yun K
First Department of Internal Medicine, School of Medicine, Fukuoka University, Japan.
J Hum Genet. 1998;43(1):49-53. doi: 10.1007/s100380050036.
To assess the relationship between insulin-like growth factor II (IGF2) and H19 gene expression at the cellular level, we have examined the distribution of IGF2 and H19 mRNA by means of an situ hybridization in hepatic malignancies consisting of hepatocellular carcinoma (HCC), cholangiocellular carcinoma (CCC), and metastatic liver cancer (MLC). In HCC, 15 of 27 tumors (56%) and 11 of 27 tumors (41%) demonstrated increased IGF2 and H19 gene expression, respectively. Of 16 HCCs with increased expression of either IGF2 or H19, 10 tumors coexpressed both transcripts at comparable levels. Moreover, the spatiotemporal distribution and the cellular localization of the two gene transcripts were almost identical, suggesting the presence of a reciprocal relation between IGF2 and H19. In addition, 5 HCCs showed increased IGF2 expression without concomitant H19 expression, whereas 1 HCC showed increased H19 expression without IGF2 transcripts. However, 11 HCCs showed no IGF2 or H19 expression. On the other hand, neither IGF2 transcripts nor H19 transcripts were detected in 2 CCCs or 10 MLCs studied. The data suggest that IGF2 and/or H19 gene expression may be characteristic of some HCCs.
为了在细胞水平评估胰岛素样生长因子II（IGF2）与H19基因表达之间的关系，我们通过原位杂交检测了由肝细胞癌（HCC）、胆管细胞癌（CCC）和转移性肝癌（MLC）组成的肝脏恶性肿瘤中IGF2和H19 mRNA的分布。在HCC中，27个肿瘤中有15个（56%）和27个肿瘤中有11个（41%）分别显示IGF2和H19基因表达增加。在16个IGF2或H19表达增加的HCC中，10个肿瘤以相当的水平共表达这两种转录本。此外，两种基因转录本的时空分布和细胞定位几乎相同，表明IGF2和H19之间存在相互关系。另外，5个HCC显示IGF2表达增加而无伴随Hl9表达，而1个HCC显示H19表达增加而无IGF2转录本。然而，11个HCC未显示IGF2或H19表达。另一方面，在所研究的2个CCC或10个MLC中均未检测到IGF2转录本或H19转录本。数据表明IGF2和/或H19基因表达可能是某些HCC的特征。
