Wood D P, Fair W R, Chaganti R S
Sloan-Kettering Institute, Department of Surgery, Memorial Hospital, Memorial Sloan-Kettering Cancer Center, New York, New York.
J Urol. 1992 Jan;147(1):274-7. doi: 10.1016/s0022-5347(17)37212-9.
The epidermal growth factor receptor has been implicated in the malignant transformation of cells because the v-erbB oncogene is a truncated form of the epidermal growth factor receptor. DNA amplification and/or mRNA overexpression of the epidermal growth factor receptor is associated with the malignant potential of several human epithelial tumors. To determine the frequency of epidermal growth factor receptor DNA amplification and mRNA overexpression in bladder cancer, we evaluated 12 bladder tumors for DNA amplification and another 14 bladder tumors for mRNA overexpression. By Southern hybridization, we found no evidence of DNA amplification or gene rearrangements in 12 bladder tumors. Five of 14 bladder tumors overexpressed epidermal growth factor receptor mRNA four to 15 fold compared to normal urothelium from the same bladder by Northern analysis. These findings suggest that epidermal growth factor receptor amplification and/or gene rearrangement occurs infrequently in bladder cancer. Epidermal growth factor receptor mRNA overexpression, however, is found in 36% of bladder tumors and may play a role in bladder tumorogenesis.
表皮生长因子受体与细胞的恶性转化有关，因为v-erbB癌基因是表皮生长因子受体的截短形式。表皮生长因子受体的DNA扩增和/或mRNA过表达与几种人类上皮肿瘤的恶性潜能相关。为了确定膀胱癌中表皮生长因子受体DNA扩增和mRNA过表达的频率，我们评估了12例膀胱肿瘤的DNA扩增情况，并评估了另外14例膀胱肿瘤的mRNA过表达情况。通过Southern杂交，我们在12例膀胱肿瘤中未发现DNA扩增或基因重排的证据。通过Northern分析，14例膀胱肿瘤中有5例的表皮生长因子受体mRNA表达量比来自同一膀胱的正常尿路上皮高4至15倍。这些发现表明，表皮生长因子受体扩增和/或基因重排在膀胱癌中很少发生。然而，在36%的膀胱肿瘤中发现了表皮生长因子受体mRNA过表达，其可能在膀胱肿瘤发生过程中起作用。
