Mellon J K, Lunec J, Wright C, Horne C H, Kelly P, Neal D E
Department of Urology, Freeman Hospital, Newcastle upon Tyne, United Kingdom.
J Urol. 1996 Jan;155(1):321-6. doi: 10.1016/s0022-5347(01)66653-9.
To study the c-erbB-2 oncogene in primary transitional cell bladder cancer.
Ninety-five patients with known clinical follow-up and epidermal growth factor receptor (EGFr) status were studied for expression of c-erbB-2 by immunostaining. Possible mechanisms underlying increased staining for c-erbB-2 protein were investigated by analyzing DNA and RNA encoding c-erbB-2.
Strong positive staining for c-erbB-2 was detected in 20 (21%) tumors, with weaker staining in a further 13 (14%). There was no correlation between increased staining for c-erbB-2 and tumor stage, grade, or EGFr status. There was a low rate of amplification of the c-erbB-2 gene (1 of 24) on Southern blotting with a higher rate of elevated c-erbB-2 mRNA (4 of 44) with dot blot hybridization. For pT1 tumors, the rate of recurrence was higher for those tumors which were positive for c-erbB-2.
c-erbB-2 oncoprotein is expressed by a significant proportion of transitional cell tumors of the bladder. In this study, the prognostic significance of c-erbB-2 expression appears limited.
研究原发性移行细胞膀胱癌中的c-erbB-2癌基因。
对95例有已知临床随访资料和表皮生长因子受体（EGFr）状态的患者，通过免疫染色研究c-erbB-2的表达。通过分析编码c-erbB-2的DNA和RNA，研究c-erbB-2蛋白染色增加的潜在机制。
在20例（21%）肿瘤中检测到c-erbB-2强阳性染色，另有13例（14%）染色较弱。c-erbB-2染色增加与肿瘤分期、分级或EGFr状态之间无相关性。Southern印迹法检测c-erbB-2基因扩增率较低（24例中有1例），斑点印迹杂交法检测c-erbB-2 mRNA升高率较高（44例中有4例）。对于pT1期肿瘤，c-erbB-2阳性的肿瘤复发率较高。
相当一部分膀胱移行细胞肿瘤表达c-erbB-2癌蛋白。在本研究中，c-erbB-2表达的预后意义似乎有限。
