Salas Alfonso Leija, Ocampo Griselda, Fariña German Garrido, Reyes-Esparza Jorge, Rodríguez-Fragoso Lourdes
Facultad de Farmacia, Universidad Autónoma del Estado de Morelos. Cuernavaca, Morelos. México.
Ann Hepatol. 2007 Jan-Mar;6(1):41-7.
Fibrosis accompanies most chronic liver disorders and is a major factor contributing to hepatic failure. Therefore, the need for an effective treatment with the aim of modifying the clinical course of this disease is evident. The aim of this work is to determine whether genistein, which has been shown to modulate the physiology and pathophysiology of liver, is able to decrease experimental liver fibrosis and cholestasis. In male Wistar rats, the common bile duct was ligated. Administration of genistein (5 microg rat-1, day-1, p.o.) began four weeks after biliary obstruction and continued for a further four weeks. The liver was used for histological and ultrastructural analysis and for collagen quantification (hydroxyproline content). The degradation of Matrigel(R) and collagen type I was determined in homogenized liver. Bilirubins and enzyme activities were measured in serum. Genistein was able to improve normal liver histology, ultrastructure, collagen content, and biochemical markers of liver damage. It also increased Matrigel(R) and collagen type I degradation. In summary, the present report shows that genistein inhibits the fibrosis and cholestasis induced by prolonged biliary obstruction in the rat. Genistein has therapeutic potential against liver fibrosis.
纤维化伴随大多数慢性肝脏疾病，是导致肝衰竭的主要因素。因此，显然需要一种有效的治疗方法来改变这种疾病的临床进程。这项工作的目的是确定已被证明可调节肝脏生理和病理生理的染料木黄酮是否能够减轻实验性肝纤维化和胆汁淤积。在雄性Wistar大鼠中，结扎胆总管。在胆道梗阻四周后开始给予染料木黄酮（5微克/大鼠，第1天，口服），并持续四周。取肝脏进行组织学和超微结构分析以及胶原定量（羟脯氨酸含量）测定。在匀浆肝脏中测定基质胶和I型胶原的降解情况。测定血清中的胆红素和酶活性。染料木黄酮能够改善正常肝脏组织学、超微结构、胶原含量以及肝损伤的生化标志物。它还增加了基质胶和I型胶原的降解。总之，本报告表明染料木黄酮可抑制大鼠长期胆道梗阻诱导的纤维化和胆汁淤积。染料木黄酮对肝纤维化具有治疗潜力。
