Kovarik Michelle L, Jacobson Stephen C
Department of Chemistry, Indiana University, Bloomington, Indiana 47405-7102, USA.
Anal Chem. 2007 Feb 15;79(4):1655-60. doi: 10.1021/ac061814m.
As fabrication techniques improve, functional fluidic devices with nanometer scale dimensions are rapidly being developed for chemical analysis. Here, we present fluid dispensing in nanochannels with injection volumes ranging from 42 aL to 4.1 fL. Devices with hybrid poly(dimethylsiloxane) and glass nanochannels, 130 nm deep and 580 nm wide or 130 nm deep and 670 nm wide, were used to evaluate two sample dispensing schemes, modified pinched and gated injections. Electrokinetic transport was achieved by applying up to 10 V directly from an analog output board without amplification, producing modest electric field strengths in the nanochannels (0.2-2 kV/cm) and enabling rapid dispensing and analysis (10-100 ms).
随着制造技术的进步，具有纳米尺度尺寸的功能性流体装置正迅速被开发用于化学分析。在此，我们展示了在纳米通道中的流体分配，进样体积范围从42阿升至4.1飞升。使用具有混合聚二甲基硅氧烷和玻璃纳米通道（深130纳米、宽580纳米或深130纳米、宽670纳米）的装置来评估两种样品分配方案，即改进的夹断进样和门控进样。通过直接从模拟输出板施加高达10伏的电压而无需放大来实现电动传输，在纳米通道中产生适度的电场强度（0.2 - 2千伏/厘米），并实现快速分配和分析（10 - 100毫秒）。
