Fiorini Gina S, Yim Moonbin, Jeffries Gavin D M, Schiro Perry G, Mutch Sarah A, Lorenz Robert M, Chiu Daniel T
Department of Chemistry, University of Washington, Seattle, WA, USA.
Lab Chip. 2007 Jul;7(7):923-6. doi: 10.1039/b702548c. Epub 2007 May 11.
Thermoset polyester (TPE) microfluidic devices were previously developed as an alternative to poly(dimethylsiloxane) (PDMS) devices, fabricated similarly by replica molding, yet offering stable surface properties and good chemical compatibility with some organics that are incompatible with PDMS. This paper describes a number of improvements in the fabrication of TPE chips. Specifically, we describe methods to form TPE devices with a thin bottom layer for use with high numerical aperture (NA) objectives for sensitive fluorescence detection and optical manipulation. We also describe plasma-bonding of TPE to glass to create hybrid TPE-glass devices. We further present a simple master-pretreatment method to replace our original technique that required the use of specialized equipment.
热固性聚酯（TPE）微流控装置先前已被开发出来，作为聚二甲基硅氧烷（PDMS）装置的替代品，其制造方式类似，都是通过复制成型法制造，但TPE装置具有稳定的表面特性，并且与一些与PDMS不相容的有机物具有良好的化学相容性。本文描述了TPE芯片制造过程中的一些改进。具体而言，我们描述了形成具有薄底层的TPE装置的方法，该装置可与高数值孔径（NA）物镜配合使用，用于灵敏的荧光检测和光学操控。我们还描述了将TPE与玻璃进行等离子体键合以制造TPE-玻璃混合装置的方法。我们进一步提出了一种简单的母版预处理方法，以取代我们原来需要使用专门设备的技术。
