Pandhal Jagroop, Wright Phillip C, Biggs Catherine A
Biological and Environmental Systems Group, Department of Chemical and Process Engineering, The University of Sheffield, Mappin Street, Sheffield S1 3JD, United Kingdom.
J Proteome Res. 2007 Mar;6(3):996-1005. doi: 10.1021/pr060460c. Epub 2007 Feb 14.
In this study, we conducted biological and technical replicate proteomic experiments using isobaric tags for relative and absolute quantification (iTRAQ), to elucidate the light adaptation strategies of Prochlorococcus marinus MED4. The MED4 strain is adapted to an oceanic environment characterized by low nutrient levels, and ever-changing light intensities. Approximately 11% of the proteome was identified, with an average coefficient of variation of iTRAQ quantification values of 0.15. Fifteen proteins were deemed to be statistically and significantly differentially expressed in changing light intensities, particularly the down-regulation of photosystem-related proteins, and the up-regulation of the stress-related chaperone GroEL in high light compared to low light.
在本研究中，我们使用等压标签相对和绝对定量（iTRAQ）技术进行了生物学和技术重复蛋白质组学实验，以阐明海洋原绿球藻MED4的光适应策略。MED4菌株适应低营养水平和不断变化的光照强度的海洋环境。我们鉴定出了约11%的蛋白质组，iTRAQ定量值的平均变异系数为0.15。有15种蛋白质被认为在光照强度变化时存在统计学上的显著差异表达，特别是与光系统相关的蛋白质下调，以及与低光照相比，高光下应激相关伴侣蛋白GroEL上调。
