Toprak Erdal, Selvin Paul R
Center for Biophysics and Computational Biology, University of Illinois, Urbana-Champaign, Urbana, Illinois 61801, USA.
Annu Rev Biophys Biomol Struct. 2007;36:349-69. doi: 10.1146/annurev.biophys.36.040306.132700.
Single-molecule biophysics has been serving biology for more than two decades. Fluorescence microscopy is one of the most commonly used tools to identify molecules of interest and to visualize biological events. Here we describe some of the most commonly used fluorescence imaging tools to measure nanoscale movements and the rotational dynamics of biomolecules.
单分子生物物理学为生物学服务已有二十多年。荧光显微镜是用于识别感兴趣分子并可视化生物事件的最常用工具之一。在此，我们描述一些最常用的荧光成像工具，用于测量生物分子的纳米级运动和旋转动力学。
