Owens K, Weglicki W B, Ruth R C, Gottwik M G, McNamara D B
Recent Adv Stud Cardiac Struct Metab. 1975;6:245-57.
Subcellular fractions from ventricular muscle of the cardiomyopathic Syrian hamster and breast muscle from the dystrophic chicken were prepared by zonal centrifugation in sucrose gradients. This tenchnique permitted the analysis of a wide spectrum of subcellular particles prior to sedimentation, thus avoiding loss of activity through particle clumping. Substantial differences in the distribution of beta-glucuronidase and p-nitrophenyl-phosphatase in zonal fractions from myopathic and control tissues provided evidence for contamination of fractions containing fragmented sarcoplasmic reticulum by lysosomes. This contamination may contribute signifantly to the altered lipid composition and Ca2+ transport function of the myopathic preparations.
通过在蔗糖梯度中进行区带离心，制备了心肌病叙利亚仓鼠心室肌和营养不良鸡胸肌的亚细胞组分。这种技术能够在沉降之前分析多种亚细胞颗粒，从而避免因颗粒聚集而导致的活性丧失。来自病变组织和对照组织的区带组分中β-葡萄糖醛酸酶和对硝基苯磷酸酶的分布存在显著差异，这为含有破碎肌浆网的组分被溶酶体污染提供了证据。这种污染可能对病变制剂中脂质组成和Ca2+转运功能的改变有重要影响。
