在直接乙醇发酵中，通过促进淀粉颗粒与武装酵母之间的表面接触来提高乙醇产量。
Enhancement of ethanol production by promoting surface contact between starch granules and arming yeast in direct ethanol fermentation.
作者信息
Khaw Teik Seong, Katakura Yoshio, Ninomiya Kazuaki, Moukamnerd Churairat, Kondo Akihiko, Ueda Mitsuyoshi, Shioya Suteaki
机构信息
Department of Biotechnology, Graduate School of Engineering, Osaka University, 2-1 Yamadaoka, Suita, Osaka 565-0871, Japan.
出版信息
J Biosci Bioeng. 2007 Jan;103(1):95-7. doi: 10.1263/jbb.103.95.
The specific ethanol production rate of raw starch by arming yeast cells displaying alpha-amylase and glucoamylase increased significantly when the cells and starch granules settled together. The specific ethanol production rate also increased when the size distribution of starch granules was almost same as that of the yeast cells. These results indicate that the surface contact between starch granules and yeast cells is important for increasing the apparent specific activity of alpha-amylase, which was the rate-limiting factor of the direct fermentation.
当展示α-淀粉酶和糖化酶的武装酵母细胞与淀粉颗粒一起沉降时，其对生淀粉的特定乙醇产率显著提高。当淀粉颗粒的尺寸分布与酵母细胞的尺寸分布几乎相同时，特定乙醇产率也会提高。这些结果表明，淀粉颗粒与酵母细胞之间的表面接触对于提高α-淀粉酶的表观比活性很重要，而α-淀粉酶是直接发酵的限速因素。
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