Zhang Yuan-Yuan, Bu Yi-Fan, Liu Jian-Zhong
Biotechnology Research Center and Biomedical Center, School of Life Sciences, Sun Yat-sen University, Guangzhou, 510275, People's Republic of China.
Folia Microbiol (Praha). 2015 Sep;60(5):393-8. doi: 10.1007/s12223-014-0371-x. Epub 2014 Dec 20.
Sucrose and molasses are attractive raw materials for industrial fermentation. Although Corynebacterium glutamicum shows sucrose-utilizing activity, sucrose or molasses is only a fraction of carbon source used in the fermentation medium in most works. An engineered C. glutamicum strain was constructed for producing L-ornithine with sucrose or molasses as a sole carbon source by transferring Mannheimia succiniciproducens β-fructofuranosidase gene (sacC). The engineered strain, C. glutamicum ΔAPE6937R42 (pEC-sacC), produced 22.0 g/L of L-ornithine with sucrose as the sole carbon source, which is on par with that obtained by the parent strain C. glutamicum ΔAPE6937R42 with glucose as the sole carbon. The resulting strain C. glutamicum ΔAPE6937R42 (pEC-sacC) produced 27.0 g/L of L-ornithine with molasses as the sole carbon source, which is higher than that obtained by the parent strain C. glutamicum ΔAPE6937R42 with glucose as the sole carbon. This strategy can be applied for developing sucrose- or molasses-utilizing industrial strains.
蔗糖和糖蜜是工业发酵中颇具吸引力的原料。尽管谷氨酸棒杆菌具有利用蔗糖的活性，但在大多数研究中，蔗糖或糖蜜只是发酵培养基中使用的碳源的一部分。通过转移产琥珀酸曼氏杆菌β-呋喃果糖苷酶基因（sacC），构建了一种工程化的谷氨酸棒杆菌菌株，用于以蔗糖或糖蜜作为唯一碳源生产L-鸟氨酸。工程菌株谷氨酸棒杆菌ΔAPE6937R42（pEC-sacC）以蔗糖作为唯一碳源时可产生22.0 g/L的L-鸟氨酸，这与亲本菌株谷氨酸棒杆菌ΔAPE6937R42以葡萄糖作为唯一碳源时获得的产量相当。所得菌株谷氨酸棒杆菌ΔAPE6937R42（pEC-sacC）以糖蜜作为唯一碳源时可产生27.0 g/L的L-鸟氨酸，这高于亲本菌株谷氨酸棒杆菌ΔAPE6937R42以葡萄糖作为唯一碳源时获得的产量。该策略可用于开发利用蔗糖或糖蜜的工业菌株。
