Lei F, Zhang X N, Wang W, Xing D M, Xie W D, Su H, Du L J
Laboratory of Pharmaceutical Sciences, Department of Biological Sciences and Biotechnology, Tsinghua University, Beijing, PR China.
Int J Obes (Lond). 2007 Jun;31(6):1023-9. doi: 10.1038/sj.ijo.0803502. Epub 2007 Feb 13.
To investigate the anti-obesity effects of the pomegranate leaf extract (PLE) in a mouse model of high-fat diet induced obesity and hyperlipidemia.
For the anti-obesity experiment, male and female ICR mice were fed with a high-fat diet to induce obesity. When the weight of the high-fat diet group was 20% higher than the normal diet group, the animals were treated with 400 or 800 mg/kg/day of PLE for 5 weeks. Body weight and daily food intake were measured regularly during the experimental period. The various adipose pads were weighed and serum total cholesterol (TC), triglyceride (TG), glucose and high-density lipoprotein cholesterol (HDL-C) were measured after 5 weeks, treatment with PLE. In the fat absorption experiment, both the normal and obese mice were given 0.5 ml lipid emulsion and PLE at a dose of 800 mg/kg at the same time. Serial serum TG levels were measured at times 1, 2, 3, 4 and 6 h after the treatment. TGs in fecal excretions were measured after the mice were orally given a lipid emulsion. Effects of PLE and its isolated compounds (ellagic acid and tannic acid) on pancreatic lipase activity were examined in vitro.
The PLE-treated groups showed a significant decrease in body weight, energy intake and various adipose pad weight percents and serum, TC, TG, glucose levels and TC/HDL-C ratio after 5 weeks treatment. Furthermore, PLE significantly attenuated the raising of the serum TG level and inhibited the intestinal fat absorption in mice given a fat emulsion orally. PLE showed a significant difference in decreasing the appetite of obese mice fed a high-fat diet, but showed no effect in mice fed a normal diet.
PLE can inhibit the development of obesity and hyperlipidemia in high-fat diet induced obese mice. The effects appear to be partly mediated by inhibiting the pancreatic lipase activity and suppressing energy intake. PLE may be a novel appetite suppressant that only affects obesity owing to a high-fat diet.
在高脂饮食诱导肥胖和高脂血症的小鼠模型中研究石榴叶提取物（PLE）的抗肥胖作用。
对于抗肥胖实验，雄性和雌性ICR小鼠喂食高脂饮食以诱导肥胖。当高脂饮食组的体重比正常饮食组高20%时，动物接受400或800毫克/千克/天的PLE治疗5周。在实验期间定期测量体重和每日食物摄入量。在给予PLE治疗5周后，称量各种脂肪垫的重量并测量血清总胆固醇（TC）、甘油三酯（TG）、葡萄糖和高密度脂蛋白胆固醇（HDL-C）。在脂肪吸收实验中，正常和肥胖小鼠同时给予0.5毫升脂质乳剂和800毫克/千克剂量的PLE。在治疗后1、2、3、4和6小时测量系列血清TG水平。在小鼠口服脂质乳剂后测量粪便排泄物中的TG。在体外检查PLE及其分离的化合物（鞣花酸和单宁酸）对胰脂肪酶活性的影响。
经过5周治疗后，接受PLE治疗的组体重、能量摄入、各种脂肪垫重量百分比以及血清TC、TG、葡萄糖水平和TC/HDL-C比值均显著降低。此外，PLE显著减弱了口服脂肪乳剂小鼠血清TG水平的升高并抑制了肠道脂肪吸收。PLE在降低高脂饮食喂养的肥胖小鼠食欲方面有显著差异，但对正常饮食喂养的小鼠没有影响。
PLE可以抑制高脂饮食诱导的肥胖小鼠肥胖和高脂血症的发展。这些作用似乎部分是通过抑制胰脂肪酶活性和抑制能量摄入来介导的。PLE可能是一种新型的食欲抑制剂，仅对高脂饮食引起的肥胖有影响。
