Bracale R, Pasanisi F, Labruna G, Finelli C, Nardelli C, Buono P, Salvatori G, Sacchetti L, Contaldo F, Oriani G
Dipartimento SPES, Università del Molise, Campobasso, Italy.
Eur J Clin Nutr. 2007 Oct;61(10):1213-9. doi: 10.1038/sj.ejcn.1602640. Epub 2007 Feb 14.
To evaluate the prevalence of beta(3)-adrenergic receptor (ADRB3) Trp64Arg polymorphism and its relationship with the metabolic syndrome in severe obesity.
Cross-sectional outpatients study.
In 265 (100 men) severely obese non-diabetic subjects and 78 (25 men) healthy volunteers, genomic DNA was isolated from peripheral leukocytes. In obese patients, plasma concentrations of leptin, lipids, glucose and insulin, the homeostasis model assessment index and blood pressure have been measured. The Trp64Arg mutation was identified with the real-time TaqMan method.
Neither genotype distribution nor allele frequency differed between the two groups. The metabolic syndrome prevalence was 59% in obese subjects, and was higher in men than in women (65 vs 55%: P=0.03). The body mass index (BMI) was related to age tertiles (beta=0.08; P<0.001; multiple linear regression) in Trp64Arg-positive obese subjects.
We confirm the high prevalence of the metabolic syndrome among severely obese subjects. ADRB3 polymorphism was significantly related to insulin resistance only in obese male subjects. Moreover, increased BMI was related to age in obese subjects with the ADRB3 polymorphism.
评估β(3)-肾上腺素能受体（ADRB3）Trp64Arg多态性的患病率及其与重度肥胖患者代谢综合征的关系。
横断面门诊研究。
在265例（100例男性）重度肥胖非糖尿病患者和78例（25例男性）健康志愿者中，从外周血白细胞中提取基因组DNA。对肥胖患者测定了瘦素、血脂、血糖和胰岛素的血浆浓度、稳态模型评估指数及血压。采用实时TaqMan法鉴定Trp64Arg突变。
两组间基因型分布和等位基因频率均无差异。肥胖患者中代谢综合征患病率为59%，男性高于女性（65%对55%：P = 0.03）。在Trp64Arg阳性肥胖患者中，体重指数（BMI）与年龄三分位数相关（β = 0.08；P < 0.001；多元线性回归）。
我们证实重度肥胖患者中代谢综合征的患病率很高。ADRB3多态性仅在肥胖男性受试者中与胰岛素抵抗显著相关。此外，在具有ADRB3多态性的肥胖受试者中，BMI升高与年龄相关。
