ADRB3 多态性 rs4994（Trp64Arg）与沙特人的体重增加和血脂异常显著相关，但 ADRB1 的 rs1801253（Arg389Gly）多态性则没有相关性。
ADRB3 polymorphism rs4994 (Trp64Arg) associates significantly with bodyweight elevation and dyslipidaemias in Saudis but not rs1801253 (Arg389Gly) polymorphism in ARDB1.
机构信息
Department of Zoology, Center for Scientific and Medical Female Colleges, King Saud University, P.O. Box 22455, Riyadh, 11495, Saudi Arabia.
Department of Obstetrics and Gynecology, Umm-Al-Qura University, P.O.Box 424, Makkah, 21955, Saudi Arabia.
出版信息
Lipids Health Dis. 2018 Mar 27;17(1):58. doi: 10.1186/s12944-018-0679-7.
BACKGROUND
In some populations, obesity and body weight related disorders show a correlation with polymorphisms in three subtypes of beta-adrenoceptor (β1, β2, and β3) [ADRB1, ADRB2 and ADRB3] genes. We scanned for the polymorphism of Arg389Gly (rs1801253) in ADRB1 and Trp64Arg (rs4994) in ADRB3 genes in Saudi population to determine association, if any, of these polymorphisms with obesity and related disorders.
METHODS
We studied 329 non-related adults (33.1% men and 66.9% women), aged 18-36 years. Anthropometric measurements were recorded, and Body mass index (BMI) and waist/hip ratio were calculated; leptin, insulin, lipidogram, and glucose concentrations were determined. ADRB1 and ADRB3 polymorphisms (Arg389Gly and Trp64Arg, respectively) were screened by DNA sequencing. The subjects were divided into three groups according to BMI: normal weight (BMI < 25 kg/m), overweight (BMI ≥25.1-29.9 kg/m) subjects, and obese (≥30 kg/m).
RESULTS
In the age-matched groups of the normal weight, overweight and obese male and female subjects, all anthropometric parameters were found to be significantly higher, and in the obese group, all biochemical parameters were significantly elevated compared to the normal weight controls. The allelic frequency of Gly389 ADRB1 did not differ amongst the three groups, whereas the frequency of Arg64 of ADRB3 gene was significantly higher in the overweight and obese subjects, compared with the normal weight subjects. In addition, subjects carrying Arg64 allele regardless of their BMI had a greater waist and hip circumference, W/H ratio, plasma cholesterol, triglyceride, LDL, leptin, insulin, and glucose level compared to those with the wild-type Trp allele.
CONCLUSION
The results of this study have shown a significant association between the Trp64Arg polymorphism in ADRB3 gene and the development of overweight and obesity in Saudi populations. It also has an influence on the levels of lipid, insulin, leptin, and glucose, whereas, Arg389Gly polymorphism in ADRB1 is not associated with overweight, obesity or dyslipidaemias in Saudis.
背景
在某些人群中，肥胖和与体重相关的疾病与β肾上腺素能受体（β1、β2 和 β3）[ADRB1、ADRB2 和 ADRB3]三种亚型的基因多态性相关。我们在沙特人群中扫描了 ADRB1 基因的 Arg389Gly（rs1801253）和 ADRB3 基因的 Trp64Arg（rs4994）多态性，以确定这些多态性与肥胖和相关疾病的任何关联。
方法
我们研究了 329 名非相关成年人（33.1%为男性，66.9%为女性），年龄在 18-36 岁之间。记录了人体测量学测量值，并计算了体重指数（BMI）和腰臀比；测定了瘦素、胰岛素、血脂谱和血糖浓度。通过 DNA 测序筛选 ADRB1 和 ADRB3 多态性（分别为 Arg389Gly 和 Trp64Arg）。根据 BMI 将受试者分为三组：正常体重组（BMI<25kg/m）、超重组（BMI≥25.1-29.9kg/m）和肥胖组（≥30kg/m）。
结果
在正常体重、超重和肥胖的男性和女性受试者的年龄匹配组中，所有人体测量参数均显著升高，而在肥胖组中，所有生化参数均显著高于正常体重对照组。ADRB1 基因的 Gly389 等位基因频率在三组之间无差异，而 ADRB3 基因的 Arg64 等位基因频率在超重和肥胖组中显著高于正常体重组。此外，无论 BMI 如何，携带 Arg64 等位基因的受试者的腰围和臀围、W/H 比值、血浆胆固醇、甘油三酯、LDL、瘦素、胰岛素和血糖水平均高于携带野生型 Trp 等位基因的受试者。
结论
本研究结果表明，ADRB3 基因的 Trp64Arg 多态性与沙特人群超重和肥胖的发生有显著关联。它还对血脂、胰岛素、瘦素和葡萄糖水平有影响，而 ADRB1 基因的 Arg389Gly 多态性与沙特人的超重、肥胖或血脂异常无关。
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