Smeyne R J, Schilling K, Robertson L, Luk D, Oberdick J, Curran T, Morgan J I
Department of Neurosciences, Roche Institute of Molecular Biology, Nutley, New Jersey 07110.
Neuron. 1992 Jan;8(1):13-23. doi: 10.1016/0896-6273(92)90105-m.
A transgenic mouse line containing a fos-lacZ fusion gene was derived in which beta-galactosidase activity identified cell populations expressing fos either constitutively or after stimulation. Seizures and light pulses induced nuclear lacZ activity in defined populations of neurons in vivo, and an array of neurotransmitters, including glutamate, induced the transgene in primary brain cultures. In unstimulated mice, the major sites of fos-lacZ expression were skin, hair follicle, and bone. fos-lacZ mice provide a new avenue for activity mapping studies based on gene expression.
构建了一个含有fos - lacZ融合基因的转基因小鼠品系，其中β - 半乳糖苷酶活性可鉴定出组成型表达fos或刺激后表达fos的细胞群体。癫痫发作和光脉冲在体内特定神经元群体中诱导核lacZ活性，并且一系列神经递质，包括谷氨酸，在原代脑培养物中诱导转基因表达。在未受刺激的小鼠中，fos - lacZ表达的主要部位是皮肤、毛囊和骨骼。fos - lacZ小鼠为基于基因表达的活性图谱研究提供了一条新途径。
