Bongso A, Fong C Y, Ng S C, Ratnam S
Department of Obstetrics and Gynaecology National University of Singapore.
Fertil Steril. 1992 Jan;57(1):129-33. doi: 10.1016/s0015-0282(16)54788-8.
To examine the fertilization rates of 48-hour unfertilized oocytes inseminated with fertile donor sperm and to evaluate the cleavage and cytogenetics of ensuing embryos.
Prospective.
Assisted reproductive technology (ART) program.
Four hundred ninety-seven unfertilized oocytes from 97 ART patients were categorized into four groups. A (zona-intact) and B (zona-free) were from patients with partial fertilization failure, whereas C (zona-intact) and D (zona-free) were total fertilization failures.
Fertilization rates in groups A and B were significantly higher than C and D (33.2% to 60.9% versus 20.0% to 48.1%; P less than 0.01). Zona-free oocytes had higher fertilization rates than zona-intact oocytes (48.1% to 60.9% versus 20.0% to 32.2%). Multiple pronuclei were high in zona-free oocytes (33.1% to 41.3%). Forty-eight to 54% of embryos generated after donor insemination had chromosome anomalies (mosaicism, aneuploidy, pulverization).
One cause of total fertilization failure appears to lie in intrinsic oocyte problems confined to the zona and oolemma. The fertilization of 48-hour unfertilized oocytes may be of some value in diagnosing fertilization failure in ART patients.
研究用可育供体精子对培养48小时未受精的卵母细胞进行授精后的受精率，并评估后续胚胎的分裂情况和细胞遗传学特征。
前瞻性研究。
辅助生殖技术（ART）项目。
97例ART患者的497个未受精卵母细胞被分为四组。A组（完整透明带）和B组（无透明带）来自部分受精失败的患者，而C组（完整透明带）和D组（无透明带）为完全受精失败的患者。
A组和B组的受精率显著高于C组和D组（33.2%至60.9% 对比 20.0%至48.1%；P<0.01）。无透明带卵母细胞的受精率高于有透明带卵母细胞（48.1%至60.9% 对比 20.0%至32.2%）。无透明带卵母细胞中多原核的比例较高（33.1%至41.3%）。供体授精后产生的胚胎中有48%至54%存在染色体异常（嵌合体、非整倍体、染色体粉碎）。
完全受精失败的一个原因似乎在于局限于透明带和卵细胞膜的卵母细胞内在问题。对培养48小时未受精的卵母细胞进行授精可能对诊断ART患者的受精失败有一定价值。
