Sengoku K, Tamate K, Takaoka Y, Horikawa M, Katayama H, Ishikawa M
Department of Obstetrics and Gynecology, Asahikawa Medical College, Japan.
Fertil Steril. 1995 Jul;64(1):122-7.
To examine the reliability of the zona-free aged (1 day old) human unfertilized oocyte sperm penetration assay for assessing sperm fertilizing ability and to determine the predictive value of this assay for subsequent subzonal insemination (SUZI) outcomes.
A total of 253 unfertilized oocytes from total fertilization failure patients and from good fertilization rate (> 70%) patients in standard IVF were inseminated with donors' spermatozoa, and penetration rates were compared in each group. Two hundred seventy-two unfertilized oocytes from total fertilization failure, poor fertilization (< 30%), and normal fertilization (> 30%) were inseminated with husbands' spermatozoa, and penetration rates were compared between the three groups. In 29 patients, the results of the zona-free assay performed in previous IVF were compared with the fertilization rates of subsequent SUZI.
In the zona-free assay using donors' spermatozoa, there was no difference in penetration rates between the two groups (109/122, 89.3% versus 114/131, 87.0%). Penetration rates using partners' spermatozoa were positively correlated with fertilization rates in standard IVF (total fertilization failure 34/75, 45.3%; poor fertilization 56/77, 72.7%; normal fertilization 108/120, 90.0%). There was a significant difference in fertilization rates after SUZI between the patients with negative and positive penetration in zona-free assay (4/53, 7.5%, versus 54/174, 31.0%).
The zona-free human oocyte assay may primarily reflect sperm fertilizing ability. This asssay also could be a reliable predictor for subsequent SUZI outcome.
检测无透明带老化（1日龄）人未受精卵母细胞精子穿透试验评估精子受精能力的可靠性，并确定该试验对后续透明带下授精（SUZI）结果的预测价值。
将来自标准体外受精中完全受精失败患者和受精率高（>70%）患者的253枚未受精卵母细胞与供体精子进行授精，并比较每组的穿透率。将来自完全受精失败、受精率低（<30%）和正常受精（>30%）的272枚未受精卵母细胞与丈夫的精子进行授精，并比较三组之间的穿透率。在29例患者中，将先前体外受精中进行的无透明带试验结果与后续SUZI的受精率进行比较。
在使用供体精子的无透明带试验中，两组的穿透率无差异（109/122，89.3%对114/131，87.0%）。使用配偶精子的穿透率与标准体外受精中的受精率呈正相关（完全受精失败34/75，45.3%；受精率低56/77，72.7%；正常受精108/120，90.0%）。无透明带试验中穿透阴性和阳性的患者在SUZI后的受精率有显著差异（4/53，7.5%对54/174，31.0%）。
无透明带人卵母细胞试验可能主要反映精子的受精能力。该试验也可能是后续SUZI结果的可靠预测指标。
