Hasan Nazarul, Siddiqui Mashiat Ullah, Toossi Zahra, Khan Saba, Iqbal Jawed, Islam Najmul
Department of Biochemistry, Faculty of Medicine, J.N. Medical College, A.M.U., Aligarh 202002, U.P., India.
Biochem Biophys Res Commun. 2007 Apr 6;355(2):471-6. doi: 10.1016/j.bbrc.2007.01.174. Epub 2007 Feb 7.
Despite of encountering a robust immune response, Mycobacterium tuberculosis (MTB) successfully survives and persists in the human host. We investigated the early regulation of MTB 85B gene by allicin in MTB-infected human monocytes. During the first 24h of infection, levels of both MTB 85B intracellular mRNA and secreted protein were significantly down-regulated by allicin in a dose-dependent manner, which was mediated by inhibition of glutathione and NF-kappaB pathway. Allicin-induced MTB 85B suppression correlated with suppression of TNF-alpha released from infected monocytes. The allicin-induced up-regulation of glutathione and IFN-gamma with simultaneous decrease in TNF-alpha supports the anti-inflammatory property of allicin by elicitation of protective immune response. Thus, allicin may prove to be valuable in the containment of MTB and therefore be useful as an adjunct in treatment of tuberculosis.
尽管会遇到强烈的免疫反应，但结核分枝杆菌（MTB）仍能成功在人类宿主中存活并持续存在。我们研究了大蒜素对感染MTB的人类单核细胞中MTB 85B基因的早期调控作用。在感染的最初24小时内，大蒜素以剂量依赖的方式显著下调了MTB 85B细胞内mRNA水平和分泌蛋白水平，这是通过抑制谷胱甘肽和NF-κB途径介导的。大蒜素诱导的MTB 85B抑制与感染单核细胞释放的TNF-α抑制相关。大蒜素诱导的谷胱甘肽和IFN-γ上调以及TNF-α同时降低，通过引发保护性免疫反应支持了大蒜素的抗炎特性。因此，大蒜素可能在控制MTB方面具有价值，因此可作为结核病治疗的辅助药物。
