Eiseman B, Norton L, Kralios N C
Surg Gynecol Obstet. 1976 Jan;142(1):21-8.
A centrifuge, as used in blood banks for washing erythrocytes, is shown to be a good perfusion chamber for hepatocyte suspensions being evaluated for potential extracorporeal liver support. The perfusate was cell-free oxygenated--pO2 300 millimeters of mercury--plasma, serum or tissue culture medium. A centrifuge that requires intermittent perfusion cannot deliver sufficient oxygen to maintain prolonged viability. A continuous flow unit can, at a flow of 800 milliliters per minute, support most functions of 100 grams of hepatocytes for six hours and some function for 12 hours. Metabolism of 2-14C tagged pyruvate to 14CO2 is the most useful sensitive measurement of liver function in this system. It is concluded that a centrifuge allowing a constant perfusion of oxygenated plasma into hepatocytes kept within the unit by centrifugal force is a suitable base for extracorporeal liver support.
血库中用于洗涤红细胞的离心机，被证明是一个很好的灌注室，可用于评估肝细胞悬浮液作为潜在体外肝脏支持的情况。灌注液为无细胞的含氧（氧分压为300毫米汞柱）血浆、血清或组织培养基。需要间歇性灌注的离心机无法提供足够的氧气来维持细胞的长期存活。一个连续流动装置，以每分钟800毫升的流速，可以在6小时内支持100克肝细胞的大部分功能，并在12小时内支持部分功能。在这个系统中，将2-¹⁴C标记的丙酮酸代谢为¹⁴CO₂是肝功能最有用的敏感测量方法。得出的结论是，一台通过离心力将含氧血浆持续灌注到单元内肝细胞中的离心机，是体外肝脏支持的合适基础。
