Mito M, Kusano M, Onishi T, Saito T, Ebata H
Gastroenterol Jpn. 1978;13(6):480-90.
Hepatocellular transplantation into the spleen was investigated as a new attempt in utilizing isolated hepatocytes to compensate for impaired liver function. Present study was undertaken to evaluate morphological and histochemical alterations up to 6 weeks following transplantation in hepatocytes transplanted into the splenic parenchyma. Light microscopic studies revealed viable hepatocellular islets in the splenic parenchyma up to 6 weeks, although minimal cytoplasmic changes were observed. Electron microscopic studies demonstrated moderate changes in organelles, which developed gradually as the time after transplantation proceeded. Distortion and fragmentation of the membranes around the cytoplasm and organelles were not recognized. Moreover, newly formed bile canaliculi and tight junctions which indicate reconstruction of hepatic plates were observed between adjacent cell membranes, and enzyme activities were detected by cytochemical determination of glucose-6-phosphatase in the hepatocytes even 6 weeks after transplantation. The transplanted hepatocytes preserved their characteristic enzyme and fine structures as hepatocytes up to 6 weeks. Our present study based on the persistence of cellular viability suggests that inoculated hepatocytes do maintain their hepatocellular functions after transplantation.
肝细胞移植到脾脏是利用分离的肝细胞来补偿肝功能受损的一种新尝试。本研究旨在评估肝细胞移植到脾实质后长达6周的形态学和组织化学变化。光镜研究显示，在长达6周的时间里，脾实质内存在有活力的肝细胞岛，尽管观察到最小的细胞质变化。电镜研究表明，细胞器有中度变化，随着移植后时间的推移逐渐发展。未发现细胞质和细胞器周围膜的扭曲和断裂。此外，在相邻细胞膜之间观察到新形成的胆小管和紧密连接，这表明肝板正在重建，甚至在移植6周后通过肝细胞中葡萄糖-6-磷酸酶的细胞化学测定检测到酶活性。移植的肝细胞在长达6周的时间里保持了其作为肝细胞的特征性酶和精细结构。我们目前基于细胞活力持续存在的研究表明，接种的肝细胞在移植后确实保持了其肝细胞功能。
