Claisse Olivier, Renouf Vincent, Lonvaud-Funel Aline
UMR oenologie, ISVV INRA-Université Victor Segalen Bordeaux2, 351 cours de la libération, 33405 Talence cedex, France.
J Microbiol Methods. 2007 May;69(2):387-90. doi: 10.1016/j.mimet.2007.01.004. Epub 2007 Jan 19.
PCR-RFLP analysis of rpoB sequences were used to identify lactic acid bacteria (LAB) species commonly isolated from wine. Strains of seven cocci and 12 lactobacilli species could be identified after single digestion with AciI for the cocci and two or three digestions (AciI, HinfI and MseI) for the rods and preceded by colonies isolation on solid selective medium and microscope observation to distinguish cocci and rods cells.
利用rpoB序列的聚合酶链反应-限制性片段长度多态性（PCR-RFLP）分析来鉴定葡萄酒中常见的乳酸菌（LAB）种类。在用AciI对球菌进行单次酶切以及对杆菌进行两次或三次酶切（AciI、HinfI和MseI）后，可鉴定出7种球菌和12种乳杆菌的菌株，在此之前需在固体选择性培养基上进行菌落分离，并通过显微镜观察来区分球菌和杆菌细胞。
