Werner Ernst R, Hermetter Albin, Prast Helmut, Golderer Georg, Werner-Felmayer Gabriele
Division of Biological Chemistry, Biocenter, Innsbruck Medical University, A-6020 Innsbruck, Austria.
J Lipid Res. 2007 Jun;48(6):1422-7. doi: 10.1194/jlr.D600042-JLR200. Epub 2007 Feb 15.
An assay was set up for glyceryl ether monooxygenase activity in tissue samples using the novel substrate 1-O-pyrenedecyl-sn-glycerol and high-performance liquid chromatographic analysis of reaction mixtures with fluorescence detection, allowing robust detection of enzymatic activity in microgram amounts of tissue homogenates. The activity partially purified from rat liver strictly depended on the presence of a tetrahydropteridine. Tetrahydrobiopterin-dependent glyceryl ether monooxygenase activity was observed in all rat tissues tested except female heart, with highest activities in liver, intestine, and cerebellum. Activity was not uniformly distributed in brain: it was higher in cerebellum than in striatum or cortex. These data demonstrate that tetrahydrobiopterin-dependent glyceryl ether monooxygenase is found not only in liver and the gastrointestinal tract but also in brain and other organs of the rat and provide an additional goal for tetrahydrobiopterin biosynthesis in these organs.
利用新型底物1-O-芘癸基-sn-甘油以及对反应混合物进行高效液相色谱荧光检测,建立了一种用于检测组织样本中甘油醚单加氧酶活性的分析方法,该方法能够在微克量的组织匀浆中可靠地检测酶活性。从大鼠肝脏部分纯化得到的该活性严格依赖于四氢生物蝶呤的存在。在除雌性心脏外的所有测试大鼠组织中均观察到了四氢生物蝶呤依赖性甘油醚单加氧酶活性,其中肝脏、肠道和小脑的活性最高。该活性在脑中的分布并不均匀:小脑的活性高于纹状体或皮质。这些数据表明,四氢生物蝶呤依赖性甘油醚单加氧酶不仅存在于大鼠的肝脏和胃肠道中,还存在于大脑和其他器官中,并且为这些器官中四氢生物蝶呤的生物合成提供了一个额外的目标。