Ishibashi T, Imai Y
J Lipid Res. 1985 Mar;26(3):393-5.
Alkylglycerol monooxygenase of rat liver microsomes was purified approximately to 97-fold with a 30% yield by procedures including affinity chromatography on chimyl alcohol-Sepharose 4B. Chimyl alcohol (1-O-hexadecylglycerol) was converted to the p-aminobenzylidene derivative and then coupled to 6-carboxyhexyl-Sepharose. The final enzyme preparation was in nearly a homogeneous state, judging from the results of sodium dodecyl sulfate-polyacrylamide disc gel electrophoresis, and it migrated to a position corresponding to an apparent molecular weight of 45,000. The results revealed that the native form of the enzyme (estimated to have a molecular weight of 400,000 as judged by Sepharose 6B column chromatography in a previous report, Ishibashi, T., and Y. Imai. 1983. Eur. J. Biochem. 132: 23-27) will polymerize to large aggregates.
通过包括在鲨肝醇-琼脂糖4B上进行亲和层析在内的步骤，大鼠肝脏微粒体的烷基甘油单加氧酶被纯化至约97倍，产率为30%。鲨肝醇（1-O-十六烷基甘油）被转化为对氨基亚苄基衍生物，然后与6-羧基己基-琼脂糖偶联。从十二烷基硫酸钠-聚丙烯酰胺圆盘凝胶电泳的结果判断，最终的酶制剂几乎处于均一状态，并且它迁移到对应于表观分子量为45,000的位置。结果表明，该酶的天然形式（根据先前的一份报告，石桥，T.，和Y.今井。1983.欧洲生物化学杂志。132: 23-27，通过琼脂糖6B柱层析判断估计分子量为400,000）会聚合形成大的聚集体。
