Maple Jodi, Vojta Lea, Soll Jurgen, Møller Simon G
Center for Organelle Research, Department of Mathematics and Natural Science, University of Stavanger, Stavanger 4036, Norway.
EMBO Rep. 2007 Mar;8(3):293-9. doi: 10.1038/sj.embor.7400902. Epub 2007 Feb 16.
In plants, chloroplast division is an integral part of development, and these vital organelles arise by binary fission from pre-existing cytosolic plastids. Chloroplasts arose by endosymbiosis and although they have retained elements of the bacterial cell division machinery to execute plastid division, they have evolved to require two functionally distinct forms of the FtsZ protein and have lost elements of the Min machinery required for Z-ring placement. Here, we analyse the plastid division component accumulation and replication of chloroplasts 3 (ARC3) and show that ARC3 forms part of the stromal plastid division machinery. ARC3 interacts specifically with AtFtsZ1, acting as a Z-ring accessory protein and defining a unique function for this family of FtsZ proteins. ARC3 is involved in division site placement, suggesting that it might functionally replace MinC, representing an important advance in our understanding of the mechanism of chloroplast division and the evolution of the chloroplast division machinery.
在植物中，叶绿体分裂是发育的一个组成部分，这些重要的细胞器通过已有的胞质质体进行二分裂产生。叶绿体通过内共生起源，尽管它们保留了细菌细胞分裂机制的一些成分来执行质体分裂，但它们已经进化到需要两种功能不同形式的FtsZ蛋白，并且失去了Z环定位所需的Min机制的一些成分。在这里，我们分析了叶绿体3（ARC3）的质体分裂成分积累和复制，并表明ARC3是基质质体分裂机制的一部分。ARC3与AtFtsZ1特异性相互作用，作为Z环辅助蛋白，为该FtsZ蛋白家族定义了独特功能。ARC3参与分裂位点定位，表明它可能在功能上替代MinC，这代表了我们对叶绿体分裂机制和叶绿体分裂机器进化理解的一个重要进展。
