Chen Dan, Huang Xiaowei, Liu Linlin, Shi Nian
Department of Toxicology, MOE Key Laboratory of Environmental and Health, Tongji Medical College of Huazhong University of Science and Technology, Wuhan 430030, Hubei, China.
J Appl Toxicol. 2007 Jul-Aug;27(4):368-72. doi: 10.1002/jat.1215.
Deltamethrin is a type II synthetic pyrethroid insecticide and is used extensively. Numerous studies have demonstrated that deltamethrin can cause severe central nervous system symptoms. The mechanism of neuro-toxicity caused by deltamethrin is still poorly understood. This study investigated the effect of deltamethrin on the mitochondria-mediated apoptosis pathway in rat brain. The cortex and hippocampus mitochondrial membrane potential, permeability transition, expression of cytochrome c and activity of cytochrome c oxidase was assayed at 5, 24, 48 and 72 h following deltamethrin treatment. The study observed that the membrane potential, change of absorbance at 540 nm (A(540)), intensities of cytochrome c in mitochondria and activity of cytochrome c oxidase in the cortex and hippocampus of treated groups were significantly lower than those of the control group. The results of the study demonstrated that deltamethrin induced a decreased mitochondrial membrane potential and increased permeability, and reduced the expression of cytochrome c, thus depressing the activity of cytochrome c oxidase significantly. It indicates that deltamethrin may have an effect on mitochondria-mediated apoptosis of nerve cells in the rat brain.
溴氰菊酯是一种II型合成拟除虫菊酯类杀虫剂，被广泛使用。大量研究表明，溴氰菊酯可导致严重的中枢神经系统症状。溴氰菊酯引起神经毒性的机制仍了解甚少。本研究调查了溴氰菊酯对大鼠脑线粒体介导的凋亡途径的影响。在溴氰菊酯处理后的5、24、48和72小时，检测了皮质和海马的线粒体膜电位、通透性转换、细胞色素c的表达以及细胞色素c氧化酶的活性。研究观察到，处理组皮质和海马的膜电位、540nm处吸光度的变化（A(540)）、线粒体中细胞色素c的强度以及细胞色素c氧化酶的活性均显著低于对照组。研究结果表明，溴氰菊酯可导致线粒体膜电位降低、通透性增加，并减少细胞色素c的表达，从而显著抑制细胞色素c氧化酶的活性。这表明溴氰菊酯可能对大鼠脑神经元细胞的线粒体介导凋亡有影响。
