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G蛋白偶联受体激酶相互作用蛋白1 RNA发夹抑制成骨细胞迁移的机制

[Mechanism of G protein coupled receptor kinase interacting protein 1 RNA hairpin inhibiting osteoblasts migration].

作者信息

Zhang Ning, Hu Zhiyi, Yin Guoyong

机构信息

Department of Orthopaedics, the First Affiliated Hospital of Nanjing Medical University, Nanjing Jiangsu, 210029, P. R. China.

出版信息

Zhongguo Xiu Fu Chong Jian Wai Ke Za Zhi. 2007 Jan;21(1):1-5.

PMID:17304992
Abstract

OBJECTIVE

To study the function and mechanism of G protein coupled receptor kinase interacting protein 1 (GIT1) RNA hairpin (GIT1-RNAh) in osteoblast migration.

METHODS

The sixth passage osteoblasts were divided into 2 groups and were infected by GIT1-RNAh (experimental group) and green fluoresence protein RNA hairpin (GFP-RNAh) (control group) adenovirus for 12 hours respectively. Each group was further classfied into two groups according to with or without platelet-drived growth factor (PDGF) stimulation. The GIT1 expression and Paxillin distribution was analyzed by immunofluorescence staining. Paxillin phosphorylation was detected by Western Blot. The localization of Paxillin was determined by co-immunofluorescence staining after transfection with cyanine fluorescence protein tagged GIT1-RNAh (CFP-GIT1-RNAh) (experimental group) and GFP-RNAh (CFP-GFP-RNAh) (control group). The role of GIT1-RNAh (experimental group) and GFP-RNAh (control group) adenovirus in osteoblasts migration was determined by wound healing assay.

RESULTS

Immunofluorescence staining results showed that the GIT1-RNAh significantly inhibited endogenous GIT1 expression, interfered Paxillin distribution. Western Blot results showed that Paxillin phosporylation was obviously inhibited in osteoblasts infected with GIT1-RNAh adenovirus (P< 0.05). The wound healing assay results showed that GIT1-RNAh adenovirus significantly inhibited osteoblast migration induced by PDGF.

CONCLUSION

GIT1-RNAh inhibits osteoblasts migration by interfering paxillin distribution and decrease Paxillin phosphorylation.

摘要

目的

研究G蛋白偶联受体激酶相互作用蛋白1（GIT1）RNA发夹（GIT1-RNAh）在成骨细胞迁移中的作用及机制。

方法

将第6代成骨细胞分为2组，分别用GIT1-RNAh（实验组）和绿色荧光蛋白RNA发夹（GFP-RNAh）（对照组）腺病毒感染12小时。每组再根据有无血小板衍生生长因子（PDGF）刺激进一步分为两组。通过免疫荧光染色分析GIT1表达和桩蛋白分布。用蛋白质免疫印迹法检测桩蛋白磷酸化。在用花青荧光蛋白标记的GIT1-RNAh（CFP-GIT1-RNAh）（实验组）和GFP-RNAh（CFP-GFP-RNAh）（对照组）转染后，通过共免疫荧光染色确定桩蛋白的定位。通过伤口愈合试验确定GIT1-RNAh（实验组）和GFP-RNAh（对照组）腺病毒在成骨细胞迁移中的作用。

结果

免疫荧光染色结果显示，GIT1-RNAh显著抑制内源性GIT1表达，干扰桩蛋白分布。蛋白质免疫印迹结果显示，感染GIT1-RNAh腺病毒的成骨细胞中桩蛋白磷酸化明显受到抑制（P<0.05）。伤口愈合试验结果显示，GIT1-RNAh腺病毒显著抑制PDGF诱导的成骨细胞迁移。

结论

GIT1-RNAh通过干扰桩蛋白分布并降低桩蛋白磷酸化来抑制成骨细胞迁移。

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