Assis Jacques Rosângela, dos Santos Freitas Lisiane, Flores Peres Valéria, Dariva Cláudio, de Oliveira José Vladimir, Bastos Caramão Elina
Instituto de Quimica, UFRGS, Av. Bento Gonçalves, 9500 Porto Alegre Rio Grande do Sul 91501-960, Brazil.
J Sep Sci. 2006 Dec;29(18):2780-4. doi: 10.1002/jssc.200600024.
The objective of this work was to investigate the extraction of Ilex paraguariensis leaves by means of three extraction techniques: pressurized liquid extraction (PLE, also called accelerated solvent extraction--ASE), maceration, and sonication. Samples of mate tea leaves were collected from an experiment conducted under agronomic control at Indfistria e Comércio de Erva-Mate Barão LTDA, Brazil. Six solvents with increasing polarities (n-hexane, toluene, dichloromethane, ethyl acetate, acetone, and methanol) were used in this investigation. Chemical analysis of the extracts was performed by GC coupled with a mass spectrometer detector. The identification and quantification were accomplished by coinjections of certified standards. The results showed that no significant differences in the qualities of the extracts were noticed regarding the extraction methods. On the other hand, the PLE technique was found to be more effective for the extractions of caffeine, phytol, palmitic, and stearic acid. The use of PLE led to a significant decrease in the total extraction time, amount of solvent consumption, and manipulation of samples compared to maceration and ultrasound-assisted extraction methods.
加压液体萃取（PLE，也称为加速溶剂萃取——ASE）、浸渍法和超声法。马黛茶树叶样本取自巴西Indfistria e Comércio de Erva-Mate Barão LTDA在农艺控制下进行的一项实验。本研究使用了六种极性递增的溶剂（正己烷、甲苯、二氯甲烷、乙酸乙酯、丙酮和甲醇）。提取物的化学分析通过气相色谱联用质谱检测器进行。通过注入认证标准品进行鉴定和定量。结果表明，就提取方法而言，提取物的质量没有显著差异。另一方面，发现PLE技术对咖啡因、叶绿醇、棕榈酸和硬脂酸的提取更有效。与浸渍法和超声辅助提取法相比，使用PLE可显著减少总提取时间、溶剂量消耗和样品处理量。
