Department of Pharmacy, Medical Faculty, University of Novi Sad, Serbia.
Molecules. 2012 Mar 2;17(3):2518-28. doi: 10.3390/molecules17032518.
A simple and rapid HPLC method for determination of chlorogenic acid (5-O-caffeoylquinic acid) in mate tea extracts was developed and validated. The chromatography used isocratic elution with a mobile phase of aqueous 1.5% acetic acid-methanol (85:15, v/v). The flow rate was 0.8 mL/min and detection by UV at 325 nm. The method showed good selectivity, accuracy, repeatability and robustness, with detection limit of 0.26 mg/L and recovery of 97.76%. The developed method was applied for the determination of chlorogenic acid in mate tea extracts obtained by ethanol extraction and liquid carbon dioxide extraction with ethanol as co-solvent. Different ethanol concentrations were used (40, 50 and 60%, v/v) and liquid CO₂ extraction was performed at different pressures (50 and 100 bar) and constant temperature (27 ± 1 °C). Significant influence of extraction methods, conditions and solvent polarity on chlorogenic acid content, antioxidant activity and total phenolic and flavonoid content of mate tea extracts was established. The most efficient extraction solvent was liquid CO₂ with aqueous ethanol (40%) as co-solvent using an extraction pressure of 100 bar.
建立并验证了一种用于测定马黛茶提取物中绿原酸（5-O-咖啡酰奎尼酸）的简单、快速的高效液相色谱法。采用等度洗脱，流动相为水-1.5%乙酸-甲醇（85:15，v/v）。流速为 0.8mL/min，检测波长为 325nm。该方法具有良好的选择性、准确性、重复性和稳健性，检测限为 0.26mg/L，回收率为 97.76%。所建立的方法用于测定乙醇提取和以乙醇为共溶剂的液态二氧化碳提取的马黛茶提取物中的绿原酸。使用不同浓度的乙醇（40、50 和 60%，v/v），并在不同压力（50 和 100 巴）和恒定温度（27±1°C）下进行液态 CO₂提取。结果表明，提取方法、条件和溶剂极性对马黛茶提取物中绿原酸含量、抗氧化活性以及总酚和类黄酮含量有显著影响。最有效的提取溶剂是液态 CO₂与水-乙醇（40%）的混合物，采用 100 巴的提取压力。
