一种用于枯草芽孢杆菌的新型冷诱导表达系统。
A novel cold-inducible expression system for Bacillus subtilis.
作者信息
Thuy Le Ai Thi, Schumann Wolfgang
机构信息
Institute of Genetics, Universität Bayreuth, D-95440 Bayreuth, Germany.
出版信息
Protein Expr Purif. 2007 Jun;53(2):264-9. doi: 10.1016/j.pep.2006.12.023. Epub 2007 Jan 9.
Production of recombinant proteins at low temperatures is one strategy to prevent formation of protein aggregates and the use of an expensive inducer such as IPTG. We report on the construction of two expression vectors both containing the cold-inducible des promoter of Bacillus subtilis, where one allows intra- and the other extracellular synthesis of recombinant proteins. Production of recombinant proteins started within the first 30min after temperature downshock to 25 degrees C and continued for about 5h.
在低温下生产重组蛋白是防止蛋白质聚集体形成以及避免使用昂贵诱导剂（如异丙基-β-D-硫代半乳糖苷，IPTG）的一种策略。我们报道了两种表达载体的构建，这两种载体均含有枯草芽孢杆菌的冷诱导des启动子，其中一种允许重组蛋白在细胞内合成，另一种则允许在细胞外合成。在温度降至25℃后的前30分钟内开始生产重组蛋白，并持续约5小时。
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