Ognianov D, Kharalambiev K H, Panova M
Vet Med Nauki. 1975;12(10):11-7.
Studied was the complement-fixation reaction in the diagnosis of the adenovirus infections in calves. It was found that the test is specific and sensitive. A virus suspension of tissue cultures was used as an antigen, its titer varying from 1:8 up to 1:16. Specific complement-fixing antibodies were detected mainly in survivals at serum dilution of up to 1:64. Specific antibodies were established in undiluted sera too taken from animals of infected herds. In some of the tested sera there were both complement-fixing and precipitating antibodies. In calves treated with a live vaccine against adenoviruses the dynamics of the complement-fixing antibodies was followed up.
研究了补体结合反应在犊牛腺病毒感染诊断中的应用。发现该试验具有特异性和敏感性。用组织培养的病毒悬液作为抗原,其效价从1:8到1:16不等。特异性补体结合抗体主要在血清稀释至1:64的存活动物中检测到。从未稀释的血清中也检测到了特异性抗体,这些血清取自感染牛群的动物。在一些检测的血清中,既有补体结合抗体,也有沉淀抗体。对用腺病毒活疫苗治疗的犊牛,追踪了补体结合抗体的动态变化。