高效液相色谱法测定羊水和母乳中的吲哚洛尔对映体：在孕妇和哺乳期妇女药代动力学中的应用

Determination of pindolol enantiomers in amniotic fluid and breast milk by high-performance liquid chromatography: applications to pharmacokinetics in pregnant and lactating women.

作者信息

Gonçalves Paulo Vinicius Bernardes, Cavalli Ricardo Carvalho, da Cunha Sergio Pereira, Lanchote Vera Lucia

机构信息

Faculdade de Ciências Farmacêuticas de Ribeirão Preto, Departamento de Análises Clínicas, Toxicológicas e Bromatológicas, Avenida do Café s/n, University of São Paulo Campus, 14040-903 Ribeirão Preto, SP, Brazil.

出版信息

J Chromatogr B Analyt Technol Biomed Life Sci. 2007 Jun 1;852(1-2):640-5. doi: 10.1016/j.jchromb.2007.01.015. Epub 2007 Jan 18.

DOI:10.1016/j.jchromb.2007.01.015

PMID:17307403

Abstract

A method for the determination of pindolol enantiomers in amniotic fluid and breast milk was developed, validated, and applied to the investigation of six pregnant women treated with rac-pindolol (10 mg/12 h). Biological samples were extracted with tert-methyl-butyl ether, and the pindolol enantiomers were resolved on a Chiralpak AD column. Amniotic fluid/plasma and milk/plasma concentrations ratios ranged from 0.4 to 4.5 and from 0.6 to 3.7, respectively, for (+)-R-pindolol and from 0.5 to 3.5 and from 1.1 to 2.8, respectively, for (-)-S-pindolol. Preliminary data suggest that amniotic fluid and breast milk are routes of fetal exposure to pindolol enantiomers.

摘要

建立了一种测定羊水和母乳中吲哚洛尔对映体的方法，对该方法进行了验证，并将其应用于对6名接受消旋吲哚洛尔（10mg/12小时）治疗的孕妇的研究。生物样品用叔丁基甲醚萃取，吲哚洛尔对映体在Chiralpak AD柱上分离。对于(+)-R-吲哚洛尔，羊水/血浆和乳汁/血浆浓度比分别为0.4至4.5和0.6至3.7；对于(-)-S-吲哚洛尔，羊水/血浆和乳汁/血浆浓度比分别为0.5至3.5和1.1至2.8。初步数据表明，羊水和母乳是胎儿接触吲哚洛尔对映体的途径。