快速电感耦合等离子体质谱法测定羊水、母乳和脑脊液中的碘。
Rapid inductively coupled plasma mass spectrometry method to determine iodine in amniotic fluid, breast milk and cerebrospinal fluid.
机构信息
Department of Clinical Laboratory, Peking Union Medical College Hospital, Peking Union Medical College & Chinese Academy of Medical Science, Beijing 100730, China.
Department of Clinical Laboratory, Peking Union Medical College Hospital, Peking Union Medical College & Chinese Academy of Medical Science, Beijing 100730, China.
出版信息
Clin Biochem. 2020 Aug;82:99-104. doi: 10.1016/j.clinbiochem.2020.03.009. Epub 2020 Mar 19.
BACKGROUND
Given that both insufficient and excess maternal iodine have adverse consequences such as poor cognitive performance, delayed physical development and increased fetal and infant mortality, the determination of maternal iodine status is very important. In this study, we established and verified a method involving inductively coupled plasma-mass spectrometry (ICP-MS) technology for the rapid determination of the amniotic fluid iodine concentration (AFIC), breast milk iodine concentration (BMIC) and cerebrospinal fluid (CSF) iodine concentration (CSFIC).
METHOD
Amniotic fluid, breast milk and CSF were collected from residual samples at Peking Union Medical College Hospital (PUMCH). The linearity, detection limit, precision, recovery, carryover and matrix effect of the testing method using ICP-MS technology were thoroughly evaluated according to the EP-10-A2 evaluation protocol approved by the Clinical and Laboratory Standards Institute (CLSI) guidelines. Furthermore, we evaluated the AFIC, BMIC and CSFIC distributions among clinical patients from PUMCH.
RESULTS
The correlation coefficient (r) was higher than 0.99 (0.995-1.000). The limit of detection (LOD) was 0.233 µg/L, and the limit of quantification (LOQ) was 0.778 µg/L. For amniotic fluid, breast milk and CSF, the assay repeatabilities were 1.5%-1.8%, 1.9%-4.0% and 1.8%-4.0%, respectively, and the within-laboratory coefficient of variations (CV%) over five days were 3.3%-9.2%, 7.2%-8.0% and 3.2%-7.8%, respectively. The recovery rates ranged from 97.7% to 109.8%. Moreover, the median concentrations of iodine in the amniotic fluid, breast milk and CSF of the patients from PUMCH were 176.3 µg/L, 136.0 µg/L, and 81.8 µg/L, respectively.
CONCLUSION
A rapid, stable and accurate method that incorporates ICP-MS technology for the determination of iodine concentration was established for amniotic fluid, breast milk and CSF in this study.
背景
由于母体碘不足和过量都会产生不良后果，如认知能力下降、身体发育迟缓以及胎儿和婴儿死亡率增加，因此确定母体碘状况非常重要。在本研究中，我们建立并验证了一种涉及电感耦合等离子体质谱（ICP-MS）技术的方法，用于快速测定羊水碘浓度（AFIC）、母乳碘浓度（BMIC）和脑脊液（CSF）碘浓度（CSFIC）。
方法
从北京协和医院（PUMCH）的剩余样本中收集羊水、母乳和 CSF。根据临床和实验室标准协会（CLSI）指南批准的 EP-10-A2 评估方案，彻底评估了使用 ICP-MS 技术的检测方法的线性、检测限、精密度、回收率、交叉污染和基质效应。此外，我们评估了 PUMCH 临床患者的 AFIC、BMIC 和 CSFIC 分布。
结果
相关系数（r）均高于 0.99（0.995-1.000）。检测限（LOD）为 0.233µg/L，定量限（LOQ）为 0.778µg/L。对于羊水、母乳和 CSF，测定重复性分别为 1.5%-1.8%、1.9%-4.0%和 1.8%-4.0%，5 天内的实验室内变异系数（CV%）分别为 3.3%-9.2%、7.2%-8.0%和 3.2%-7.8%。回收率范围为 97.7%-109.8%。此外，PUMCH 患者的羊水、母乳和 CSF 中碘的中位数浓度分别为 176.3µg/L、136.0µg/L 和 81.8µg/L。
结论
本研究建立了一种快速、稳定、准确的 ICP-MS 技术测定羊水、母乳和 CSF 中碘浓度的方法。
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