Bogdanov G N, Shmonina V M
Vopr Onkol. 1975;21(4):28-32.
Under study was the kinetic growth of three hepatomas-22A, 60 and 46, characterized by a various degree of differentiation. There was found a correlation between the degree of differentiation and parameters of the hepatomas growth. For the hepatomas a correlation between the DNA content and rate of growth was observed, for hepatoma 46 a value of the DNA content proved to be near to that of normal liver. Moreover, in a regenerating liver the DNA content is the same as normal, that indicates the difference between normal and neoplastic actively proliferating tissues. The processes of DNA synthesis, studied by thymidine-C14 incorporation, showed a linear correlation with the rate of growth in regenerating, normal and neoplastic tissues. The ratio RNA/DNA in tumors is regularly decreased with respect to normal liver (a linear correlation between RNA/DNA and the maximum rate of growth). In a regenerating liver the ratio is high. Variations in the ratio RNA/DNA reflect changes in the functional activity and related disorders in the metabolism of nucleic acids in hepatomas. There was found a suppressed DNA decomposition in autolysis of homogenates from hepatoma tissues. It is essential to note that in a minimum deviated hepatoma some inhibition of the DNA decomposition is the only observed disorder in the nucleic acids metabolism. Spin-labelled DNA preparations of tissues under study were obtained. The presence of structural differences between separate DNA samples has been demonstrated.
对三种分化程度不同的肝癌——22A、60和46的生长动力学进行了研究。发现分化程度与肝癌生长参数之间存在相关性。观察到肝癌的DNA含量与生长速率之间存在相关性，肝癌46的DNA含量值与正常肝脏相近。此外，再生肝中的DNA含量与正常肝脏相同，这表明正常组织和肿瘤性活跃增殖组织之间存在差异。通过胸腺嘧啶核苷-C14掺入研究的DNA合成过程显示，其与再生组织、正常组织和肿瘤组织的生长速率呈线性相关。肿瘤中RNA/DNA的比值相对于正常肝脏有规律地降低（RNA/DNA与最大生长速率之间呈线性相关）。再生肝中的该比值较高。RNA/DNA比值的变化反映了肝癌功能活性的变化以及核酸代谢中的相关紊乱。在肝癌组织匀浆的自溶过程中发现DNA分解受到抑制。必须指出的是，在最小偏离型肝癌中，DNA分解的某种抑制是在核酸代谢中观察到的唯一紊乱现象。获得了所研究组织的自旋标记DNA制剂。已证明不同DNA样品之间存在结构差异。
