Ye Han-Qing, Chen Song-Lin, Sha Zhen-Xia, Liu Yang
Key Lab For Sustainable Utilization of Marine Fisheries Resources, Ministry of Agriculture, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao, 266071, China.
Mar Biotechnol (NY). 2007 Mar-Apr;9(2):262-72. doi: 10.1007/s10126-006-6093-6. Epub 2007 Feb 19.
Myostatin (MSTN) is a member of the transforming growth factor-beta (TGF-beta) superfamily that functions as a negative regulator of skeletal muscle development and growth in mammals. However, few reports are available about the structure and function of MSTN in teleost. Here, the MSTN gene was cloned from sea perch (Lateolabrax japonicus) by homology cloning and genomic walking. In the 4873-bp genomic sequence, three exons, two introns, and 5' and 3' flanking sequences were identified. The sea perch MSTN gene encodes a 374-amino acid protein, including a signal peptide, conserved cysteine residues, and a RXXR proteolytic cleavage domain. Expression analysis of MSTN revealed that MSTN was highly expressed in eyes, brain, and muscle; intermediately in intestine; and weakly in gill, spleen, liver, and heart. It was demonstrated that MSTN mRNA was highly expressed in embryonic stem cell line (LJES1), but it was undetectable in several types of somatic cell lines from sea perch, including fibroblast-like cell, epithelioid cell, and lymphocyte-like cell. Further, it was demonstrated that the 5' flanking region of the MSTN gene can drive the expression of green fluorescent protein (GFP) reporter gene in LJES1 cells and transgenic zebrafish (Danio rerio). This is the first report on the expression profile of MSTN gene in various types of cell cultures.
肌肉生长抑制素（MSTN）是转化生长因子-β（TGF-β）超家族的成员，在哺乳动物中作为骨骼肌发育和生长的负调节因子发挥作用。然而，关于硬骨鱼中MSTN的结构和功能的报道较少。在此，通过同源克隆和基因组步移从海鲈（Lateolabrax japonicus）中克隆了MSTN基因。在4873 bp的基因组序列中，鉴定出三个外显子、两个内含子以及5'和3'侧翼序列。海鲈MSTN基因编码一种374个氨基酸的蛋白质，包括一个信号肽、保守的半胱氨酸残基和一个RXXR蛋白水解切割结构域。MSTN的表达分析表明，MSTN在眼睛、大脑和肌肉中高度表达；在肠道中中度表达；在鳃、脾脏、肝脏和心脏中弱表达。结果表明，MSTN mRNA在胚胎干细胞系（LJES1）中高度表达，但在海鲈的几种体细胞系中未检测到，包括成纤维样细胞、上皮样细胞和淋巴细胞样细胞。此外，结果表明MSTN基因的5'侧翼区域可驱动绿色荧光蛋白（GFP）报告基因在LJES1细胞和转基因斑马鱼（Danio rerio）中的表达。这是关于MSTN基因在各种细胞培养类型中的表达谱的首次报道。
