Chen Yan, Dai Ti-Jun, Zeng Yin-Ming
Jiangsu Province Key Laboratory of Anesthesiology, Xuzhou Medical College and Jiangsu Province Institute of Anesthesiology, Xuzhou City, Jiangsu Province, China.
Basic Clin Pharmacol Toxicol. 2007 Mar;100(3):165-9. doi: 10.1111/j.1742-7843.2006.00031.x.
The present study was designed to investigate the role of strychnine-sensitive glycine receptors in analgesia induced by emulsified inhalation anaesthetics. After having established the mice model of analgesia by intraperitoneal or subcutaneous injections of appropriate doses of ether, enflurane, isoflurane or sevoflurane, we injected different doses of strychnine intrathecally and then observed the effects on the tail-flick latency using the tail-withdrawal test and the writhing times and acetic acid-induced writhing test. In the tail-withdrawal test, all four emulsified inhalation anaesthetics (intraperitoneally) significantly increased the tail-flick latency (P < 0.01) compared with baseline, and the increase of tail-flick latency induced by four emulsified inhalation anaesthetics can be abolished by intrathecally injected strychnine. In the acetic acid-induced writhing test, writhing times inhibition induced by subcutaneous administration of four emulsified inhalation anaesthetics was not effected by intrathecal strychnine (0.1, 0.2 and 0.4 microg). The data presented in this study suggest that glycine receptors are specifically involved in mediating the analgesic effect of ether, enflurane, isoflurane and sevoflurane on thermal-induced nociception but not chemically induced nociception.
本研究旨在探讨士的宁敏感型甘氨酸受体在乳化吸入麻醉药诱导的镇痛中的作用。通过腹腔或皮下注射适当剂量的乙醚、恩氟烷、异氟烷或七氟烷建立小鼠镇痛模型后，我们鞘内注射不同剂量的士的宁，然后使用甩尾试验观察对甩尾潜伏期的影响，并使用扭体试验观察扭体次数和乙酸诱导的扭体试验。在甩尾试验中，与基线相比，所有四种乳化吸入麻醉药（腹腔注射）均显著增加了甩尾潜伏期（P < 0.01），鞘内注射士的宁可消除四种乳化吸入麻醉药诱导的甩尾潜伏期增加。在乙酸诱导的扭体试验中，鞘内注射士的宁（0.1、0.2和0.4微克）对皮下注射四种乳化吸入麻醉药诱导的扭体次数抑制没有影响。本研究中的数据表明，甘氨酸受体特异性参与介导乙醚、恩氟烷、异氟烷和七氟烷对热诱导伤害性感受的镇痛作用，但不参与化学诱导的伤害性感受。
