Department of Anesthesiology, the Affiliated People's Hospital of Jiangsu University and the First People's Hospital of Zhenjiang, Zhenjiang, Jiangsu, 212002, PR China.
Pharmacol Rep. 2011;63(4):949-55. doi: 10.1016/s1734-1140(11)70610-7.
In the present study, the role of kainate (KA) receptors in hypnosis and analgesia induced by emulsified inhalation anesthetics was investigated. A mouse model of hypnosis and analgesia was established by an intraperitoneal injection of emulsified enflurane, isoflurane or sevoflurane. We intracerebroventricularly (icv) or intrathecally (it) administered KA, a KA receptor agonist to mice. The effects of the KA on the sleep time were observed using a hypnosis test, and the tail-withdrawal latency was analyzed using the tail-withdrawal test. In the hypnosis test, KA (2.5, 5 or 10 ng; icv administered) treatment had no distinctive effects on the sleep time of mice treated with emulsified inhalation anesthetics. In the tail-withdrawal test, KA (0.2, 0.4 or 0.8 ng; it administered) treatment significantly and dose-dependently decreased the tail-withdrawal latency of mice treated with emulsified anesthetics. These results suggested that KA receptors may modulate the analgesic but not hypnotic effects induced by emulsified enflurane, isoflurane or sevoflurane.
在本研究中，研究了红藻氨酸（KA）受体在乳化吸入麻醉诱导的催眠和镇痛中的作用。通过腹腔内注射乳化的恩氟烷、异氟烷或七氟醚建立了催眠和镇痛的小鼠模型。我们向小鼠脑室内（icv）或鞘内（it）给予 KA，一种 KA 受体激动剂。使用催眠试验观察 KA 对睡眠时间的影响，并使用尾部退缩试验分析尾部退缩潜伏期。在催眠试验中，KA（2.5、5 或 10 ng；icv 给药）处理对乳化吸入麻醉处理的小鼠的睡眠时间没有明显影响。在尾部退缩试验中，KA（0.2、0.4 或 0.8 ng；it 给药）处理显著且剂量依赖性地降低了乳化麻醉处理的小鼠的尾部退缩潜伏期。这些结果表明，KA 受体可能调节乳化恩氟烷、异氟烷或七氟醚诱导的镇痛而不是催眠作用。
