Department of Surgery, Albert Einstein College of Medicine/Montefiore Medical Center, Bronx, NY, USA.
Wound Repair Regen. 1996 Oct;4(4):461-9. doi: 10.1046/j.1524-475X.1996.40410.x.
We have previously reported that local application of viable Staphylococcus aureus dramatically accelerates wound healing, but viable Staphylococcus epidermidis does not. Because the S. aureus effect occurred in the absence of infection and because the cell walls of the two bacterial species differ, we hypothesized that nonviable S. aureus, its cell wall, and its cell wall component(s) would accelerate healing. Nonviable S. aureus was prepared by chemical and physical means, and its cell wall and peptidoglycan was prepared from heat-killed cultures. In a large number of experiments, nonviable S. aureus (independent of the strain's protein A content), its cell wall, and peptidoglycan when instilled locally at the time of wounding each significantly increased the breaking strength of rat skin incisions (tested both in the fresh state and after formalin fixation). These agents also enhanced subcutaneous polyvinyl alcohol sponge reparative tissue collagen accumulation, generally by a factor of two. Histologic features of treated and control incisions were similar. In contrast, the reparative tissue of treated sponges contained more neutrophils, macrophages, capillaries, and collagen. These experimental data thus confirm our previous studies, as well as our hypothesis, and extend these observations of enhanced wound healing to specific fractions of the bacterial cell wall.
我们之前曾报道过，局部应用有活力的金黄色葡萄球菌可显著加速伤口愈合，但表皮葡萄球菌则不行。由于金黄色葡萄球菌的作用发生在没有感染的情况下，而且这两种细菌的细胞壁不同，我们假设无活力的金黄色葡萄球菌、其细胞壁及其细胞壁成分会加速愈合。通过化学和物理手段制备无活力的金黄色葡萄球菌，并从热杀死的培养物中制备其细胞壁和肽聚糖。在大量实验中，局部应用于创伤时的无活力金黄色葡萄球菌（与菌株的蛋白 A 含量无关）、其细胞壁和肽聚糖均显著增加了大鼠皮肤切口的断裂强度（在新鲜状态和福尔马林固定后均进行了测试）。这些药物还增强了皮下聚乙烯醇海绵修复组织胶原的积累，通常增加两倍。处理和对照切口的组织学特征相似。相比之下，处理过的海绵的修复组织中含有更多的中性粒细胞、巨噬细胞、毛细血管和胶原。这些实验数据证实了我们之前的研究以及我们的假设，并将这些观察到的增强伤口愈合的现象扩展到细菌细胞壁的特定部分。
