Department of Surgery,Albert Einstein College of Medicine/Montefiore Medical Center, Bronx, NY, USA.
Wound Repair Regen. 1996 Oct;4(4):470-6. doi: 10.1046/j.1524-475X.1996.40411.x.
Molecular mechanisms involved in wound healing acceleration by Staphylococcus aureus peptidoglycan were investigated with the use of polyvinyl alcohol sponges implanted under the dorsal skin of rats. Total collagen and RNA content and messenger RNA levels of alpha1(I) and alpha1(III) procollagen, transforming growth factor-beta1, and matrix metalloproteinase-1 were analyzed in saline solution- and S. aureus peptidoglycan-inoculated sponges at 4, 7, 14, and 21 days after implantation. S. aureus peptidoglycan-inoculated sponges on the fourth and seventh post-operative day were surrounded and penetrated by a thick capsule of reparative connective tissue. They were considerably heavier and contained more collagen and total RNA than saline solution-inoculated sponges. Histologically, the S. aureus peptidoglycan-inoculated sponges early on contained a denser infiltrate of polymorphonuclear cells than saline solution-inoculated sponges, and later fibroblasts, macrophages, collagen, and newly formed blood vessels were more abundant in the S. aureus peptidoglycan sponges. Matrix metalloproteinase-1 messenger RNA expression was elevated at 4 days in both sponge types. However, although matrix metalloproteinase-1 mRNA levels decreased to undetectable levels by 14 days in saline solution-inoculated sponges, they remained elevated throughout the 21-day study period in S. aureus peptidoglycan-inoculated sponges. No other significant differences in the parameters analyzed were detected. These results suggest that S. aureus peptidoglycan induces an accelerated but normal wound healing process in which the markedly increased early deposition of connective tissue is rapidly remodeled likely because of a sustained expression of matrix metalloproteinase-1.
采用聚乙烯醇海绵植入大鼠背部皮肤下的方法，研究了金黄色葡萄球菌肽聚糖加速伤口愈合的分子机制。在植入后第 4、7、14 和 21 天，用生理盐水和金黄色葡萄球菌肽聚糖接种的海绵中分析总胶原蛋白和 RNA 含量以及α1（I）和α1（III）前胶原、转化生长因子-β1 和基质金属蛋白酶-1 的信使 RNA 水平。植入后第 4 和第 7 天，金黄色葡萄球菌肽聚糖接种的海绵被修复性结缔组织的厚包膜包围和穿透。它们比生理盐水接种的海绵重得多，并且含有更多的胶原蛋白和总 RNA。组织学上，金黄色葡萄球菌肽聚糖接种的海绵在早期比生理盐水接种的海绵含有更密集的多形核细胞浸润，而在后期，成纤维细胞、巨噬细胞、胶原蛋白和新形成的血管在金黄色葡萄球菌肽聚糖海绵中更为丰富。两种海绵类型的基质金属蛋白酶-1 信使 RNA 表达在第 4 天均升高。然而，尽管生理盐水接种的海绵中基质金属蛋白酶-1 mRNA 水平在第 14 天降至无法检测的水平，但在金黄色葡萄球菌肽聚糖接种的海绵中整个 21 天研究期间仍保持升高。在分析的参数中没有发现其他显著差异。这些结果表明，金黄色葡萄球菌肽聚糖诱导加速但正常的伤口愈合过程，其中明显增加的早期结缔组织沉积迅速重塑，可能是由于基质金属蛋白酶-1 的持续表达。
