Seward David J, Cubberley Gabrielle, Kim Soojin, Schonewald Matt, Zhang Lian, Tripet Brian, Bentley David L
Department of Biochemistry and Molecular Genetics, University of Colorado School of Medicine, University of Colorado Health Sciences Center, MS8101, P.O. Box 6511, Aurora, Colorado 80045, USA.
Nat Struct Mol Biol. 2007 Mar;14(3):240-2. doi: 10.1038/nsmb1200. Epub 2007 Feb 18.
Histone H3 Lys4 trimethylation (H3-K4me3) is a conserved mark of actively transcribed chromatin. Using a conditional mutant of the yeast H3-K4 methyltransferase, Set1p, we demonstrate rapid turnover of H3-K4me3 and H3-K4me2 in vivo and show this process requires Yjr119Cp, of the JARID1 family of JmjC proteins. Ectopic overexpression of mouse Jarid1B, a Yjr119Cp homolog, greatly diminished H3-K4me3 and H3-K4me2 in HeLa cells, suggesting these proteins function as K4 demethylases in vivo.
组蛋白H3赖氨酸4三甲基化（H3-K4me3）是活跃转录染色质的一种保守标记。利用酵母H3-K4甲基转移酶Set1p的条件性突变体，我们证明了体内H3-K4me3和H3-K4me2的快速周转，并表明这一过程需要JmjC蛋白JARID1家族的Yjr119Cp。小鼠Jarid1B（一种Yjr119Cp同源物）的异位过表达极大地减少了HeLa细胞中的H3-K4me3和H3-K4me2，这表明这些蛋白在体内作为K4去甲基化酶发挥作用。
